On-Bone Fixation of Free Gingival Graft Induces the Expression of Osteogenic Differentiation Markers in the Mandible of Miniature Pigs.

Purpose: To study the distribution of markers of mesenchymal stem cells (clusters of differentiation-29 and -44), osteoblasts (osteocalcin), and transforming growth factor-β1 in the mandibular tissue of miniature pigs after on-bone fixation of a free gingival graft.

Materials And Methods: Six outbred male miniature pigs 2 years of age were used in the study. In the external surface of the mandible, the periosteum was excised from all over the free gingival graft fixation area.

The effect of calcium phosphate biodegradable coatings of titanium implants on cell differentiation and apoptosis in rat bone tissue after experimental fracture.

Background: The effectiveness of bone repair is determined by the balance of proliferative and destructive factors in the fracture union site. It can be enhanced by using various nanostructured materials possessing osteoinductive properties, in particular titanium implants with biodegradable calcium phosphate coatings. The effects of these coatings on the state of stem cells, their differentiation and distribution in the repair zone is unknown.

The topography and proliferative activity of cells immunoreactive to various growth factors in rat femoral bone tissues after experimental fracture and implantation of titanium implants with bioactive biodegradable coatings.

Background: Biodegradable implant coatings promote proliferation and expression of BMP-2, VEGF, and TGF-β2 genes and enhance BMP-2, VEGF, and TGF-β2 regulatory effects at different stages of reparative osteogenesis.

Objective: To study the topography and ratio of PCNA-, VEGF-, BMP-2-, and TGF-β2-immunoreactive cells in rat femoral bone after closed fracture and implantation of titanium implants with biodegradable calcium phosphate and hydroxyapatite coatings.

Methods: Standard titanium implant screws and similar implants with bioactive coatings were used.