Publications by authors named "Sergey Khomutov"
Article Synopsis
- Pregnenolone and progesterone are crucial steroid hormones in mammals, and new recombinant strains are needed for more efficient microbiological production from sterols.
- A new strain of Mycolicibacterium smegmatis was developed to express steroidogenic enzymes from the bovine adrenal cortex, leading to the production of progesterone and pregnenolone from cholesterol.
- The study demonstrated successful transformation of sterols under optimized conditions, yielding high-purity crystalline pregnenolone, which enhances the potential for efficient hormone production using microbial platforms.
Engineered mutants of Mycolicibacterium spp. are known producers of valuable steroid synthons with C or C skeleton. Here we describe a method for site-directed mutagenesis of Mycolicibacterium neoaurum strains, bioconversion from phytosterol, and selective purification of C steroid 24-norchol-4-ene-3,22-dione (24-NCED) and C steroid 20-hydroxymethylpregn-4-ene-3-one (20-HMP).
View Article and Find Full Text PDFTwo-step one-pot microbial transformation enables obtaining of valuable steroids that are difficult to produce chemically. Here we describe a method for obtaining 11α-hydroxyandrost-4-ene-3,17-dione (11α-HAD) from cheap and available natural sterols (phytosterols or cholesterol).11α-HAD is a primary adrenal steroid in mammals and also a key precursor in the syntheses of halogenated corticoids.
View Article and Find Full Text PDFThe synthesis of 3beta-hydroxy-androsta-5,7-dien-17-one from 3beta-hydroxy-androst-5-en-17-one (dehydroepiandrosterone, DHEA) via microbial 7alpha-hydroxylation has been accomplished. At the first stage, 3beta,7alpha-dihydroxy-androst-5-en-17-one was obtained in high yield (71.2%) using a strain of Gibberella zeae VKM F-2600, which was first applied for DHEA conversion.
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