Publications by authors named "Sergey Cherkasov"

Intermicrobial interactions play a key role in the regulation of microbial populations and the colonization of various ecological niches. In the present study, we assessed the effect of cell-free supernatants (CFSs) from the vaginal isolates Corynebacterium amycolatum ICIS 53 and Corynebacterium amycolatum ICIS 82 on urogenital test strain biofilm formation of Staphylococcus aureus. Our studies showed that the CFSs of both C.

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We proposed the nitrone-oxaziridine rearrangement as a novel method for photochemical activation for the homolysis of alkoxyamine in nitroxide-mediated polymerization. The photoisomerization of the aldo-/ketonitrone-group into the oxaziridine one in 2,5-dihydroimidazole 3-oxide-based alkoxyamines was studied; the products of photolysis have been identified, and quantum yields were measured. Conversion of the nitrone group into the oxaziridine one was found to decrease the activation energy of alkoxyamine homolysis by 10 kJ mol.

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Biofilm formation is an important factor in the development of antibiotic resistance and chronic infection. In this study, we demonstrated that the cell-free supernatant of vaginal isolates of C. amycolatum caused a reduction in biofilm formation, destroyed the preformed biofilms, altered the cell surface properties and reduced the production of exopolysaccharides in clinical isolates of P.

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The use of 2,2,5,5-tetramethyl-3-imidazoline-3-oxide-1-oxyl (1) as a controlling agent in Nitroxide Mediated Polymerization allows for activation of alkoxyamine homolysis by 1,3-dipolar cycloaddition of a vinyl monomer [Edeleva et al. Chem. Commun.

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Article Synopsis
  • Corynebacterium amycolatum ICIS 99, isolated from healthy women's vaginal smears, demonstrated positive antimicrobial potential, prompting a genome analysis.
  • The draft genome is 2,532,503 bp long, containing 2186 coding sequences with a G + C content of 59.0%, and lacks true virulence factors.
  • It has genes that help it survive in the vaginal environment and produce beneficial metabolites, suggesting its potential as a safe probiotic strain; the genomic data is available in major databases.
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  • * Genome sequencing of three vaginal strains revealed their ability to produce metabolites that enhance the fighting power of beneficial bacteria against harmful microorganisms.
  • * These strains show genes that help them survive in the vaginal environment and have potential as probiotics to prevent pathogenic growth and support healthy colonization.
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The bacterial pathogen , which causes enteritis, has a broad host range and extensive environmental longevity. In water and soil, Salmonella interacts with protozoa and multiplies inside their phagosomes. Although this relationship resembles that between and mammalian phagocytes, the interaction mechanisms and bacterial genes involved are unclear.

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Three alkoxyamines based on imidazoline radicals with a pyridine functional group-potential initiators of nitroxide-mediated, controlled radical polymerization-were synthesized. Electron Paramagnetic Resonance (EPR) measurements reveal biexponential kinetics for the thermolysis for diastereomeric alkoxyamines and monoexponential kinetics for an achiral alkoxyamine. For comparison, the thermolysis of all three alkoxyamines was studied by NMR in the presence of three different scavengers, namely tetramethylpiperidine-N-oxyl (TEMPO), thiophenol (PhSH), and β-mercaptoethanol (BME), and detailed analysis of products was performed.

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It is known that the rumen microbiome directly or indirectly contributes to animal production, and may be a prospective target for mitigation of greenhouse gas emissions [1]. At the same time, feed types and components of diet can influence the composition of the rumen microbiome [2,3]. Fluctuations in the composition of the digestive tract microbiota can alter the development, health, and productivity of cattle [4].

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is an ubiquitous pathogen throughout the world causing gastroenteritis in humans and animals. Survival of pathogenic bacteria in the external environment may be associated with the ability to overcome the stress caused by starvation. The bacterial response to starvation is well understood in laboratory cultures with a sufficiently high cell density.

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Because the C-ON bond homolysis rate constant is an essential parameter of alkoxyamine reactivity, it is especially important to tune without a major alteration of the structure of the molecule. Recently, several approaches have become known, , protonation of functional groups and formation of metal complexes. In this paper, coordination reactions of [Zn(hfac)(HO)] with a series of new SG1-based alkoxyamines affording complexes with different structures are presented.

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Article Synopsis
  • - Ciliates, particularly Paramecium, are common aquatic protists that feed on bacteria and can be easily grown in labs, but their associated prokaryotic communities haven't been studied much.
  • - Researchers used high-throughput sequencing of the 16S rRNA gene to analyze the microbiomes of various Paramecium strains from different locations, looking for biases in HTS results based on DNA library preparation.
  • - The study found significant differences in the prokaryotic composition and bacterial diversity of the microbiomes related to ciliates and their environments, with some strains hosting potentially harmful bacteria.
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  • - This study investigates the link between dental caries and bronchial asthma severity in children, focusing on differences in the oral microbiome of asthmatic kids with and without dental caries using 16S rDNA sequencing.
  • - Dental plaque samples were taken from children and analyzed, revealing no significant differences in the overall bacterial community composition between both groups, although certain species were more prevalent in specific cases.
  • - The findings showed a higher abundance of the genus Veillonella in caries-affected children, suggesting its role in dental caries development, while potential respiratory pathogens were found in the mouths of both asthmatic groups.
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The primary objective of the present study was to review the impact of Cd exposure on gut microbiota and intestinal physiology, as well as to estimate whether gut may be considered as the target for Cd toxicity. The review is based on literature search in available databases. The existing data demonstrate that the impact of Cd on gut physiology is two-sided.

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ICIS 9 was isolated from a vaginal smear of a healthy woman. Here, we report the draft genome sequence of ICIS 9, which will be useful for further studies of specific genetic features of this strain and for understanding its probiotic properties.

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This report describes the draft genome sequence of Corynebacterium amycolatum strain ICIS 53, isolated from the reproductive tract of a healthy woman. The size of the genome was 2,460,257 bp (58.98% G+C content).

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The study was conducted to examine the effect of zinc nanoparticles on survival of worms Eisenia fetida and composition of the gut microflora. Analysis of the survival data has shown that the introduction of high doses of the nanoparticles causes death of worms in the second group with 35 % mortality rate and activates protective mechanisms realized as mucous film. DNA from the worm guts was extracted and 16S metagenomic sequencing was fulfilled using MiSeq (Illumina).

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