Production of the growth factors GM-CSF, G-CSF, and VEGF by human peripheral blood cells induced with metal complexes of human serum γ -globulin formed with copper or zinc ions.

As it was established in our previous studies, the proteins of human serum γ-globulin fraction could interact with copper or zinc ions distributed in the periglobular space, form metal complexes, and become able to perform effector functions differing due to the conformational shifts from those mediated by them in native conformation of their Fc regions. In the present work we have evaluated ability of the γ-globulin metal complexes formed with copper or zinc ions in the conditions like to the physiological ones to induce production or to regulate induction in the culture of freshly isolated human peripheral blood cells (PBC) of granulocyte (G) and granulocyte-macrophage (GM) colony-stimulating factors (CSF) as well as of vascular endothelial growth factor (VEGF). The γ-globulin metal complexes formed with both copper and zinc ions were found to similarly reduce production of GM-CSF, G-CSF, and VEGF induced in normal human PBC cultures by the control γ-globulins or by copper and zinc ions used alone.

Possibility for Combination of Human rIFN-alpha and rIFN-beta in Complex Immune Therapy of Multiple Sclerosis.

Six patients with an evident multiple sclerosis (MS) were observed in dynamics of long term immune therapy based on administration of rIFN preparations. One half of them received the therapy with rIFN-alpha. Three other patients received rIFN-beta administration that was interrupted, when sensitivity of blood cells to in vitro IFN-beta action was decreased; the trial was continued by rIFN-alpha courses.