Corrigendum: HPLC for at-line reaction monitoring and purification improves yield and purity of tRNA.

[This corrects the article DOI: 10.3389/fmolb.2024.

HPLC for at-line reaction monitoring and purification improves yield and purity of tRNA.

Engineered transfer RNA is an emerging therapeutic modality, particularly suited to treatment of diseases caused by genetic disorders based on premature termination codons, frameshifts, or missense mutations. It is also extensively used in reprogramming of translation systems to generate non-canonical amino acid-containing proteins and peptides, such as in mRNA display. Due to its length, chemical synthesis of tRNA is challenging and production of engineered tRNA at scale is currently limited to transcription from a DNA template.