Measurement of NK cells' cytotoxicity and IFN production as square of the area under the functional curve.

Integral parameters of NK cell cytotoxicity and IFN production measured as square of the area under the cytotoxic and IFN titer curves were correlated with each other. The NK and IFN parameters were also calculated by the standard method using effector : target cell (ET) ratio and correlated with each other again. It was shown that NK cell cytotoxicity and IFN production by normal human PBL reacting against K562 targets correlated positively.

Detection of a Negative Correlation between NK Cell Cytotoxicity and IFN Production in Healthy Individuals.

As an extension of our investigation of the mechanisms of regulation of NK cell activity we have compared the integral parameters of NK cell cytotoxicity (IPC) and IFN production (IPIP) measured as the square of the area under the cytotoxic and IFN titer functional curves. They were compared with the parameters obtained by the standard effector : target cell (E:T) ratio method. The calculations were done in groups of healthy individuals, PBL of which are distinguished by the levels of NK cell cytotoxicity and IFN production measured in the reaction of natural cytotoxicity.

Long-Term Study of Interferon System State in Patients with Multiple Sclerosis Received the Individual Immune Therapy with Human Recombinant IFN-alpha.

Three patients with an evident multiple sclerosis (MS) were observed in a dynamics of long-term immune therapy based on administration of rIFN-alpha preparation. Production of IFN-alpha and IFN-gamma by induced peripheral blood leukocytes (PBL) of the patients was tested simultaneously with detection of a response of PBL to in vitro IFN-alpha and IFN-beta priming action every 10th day of observation. It has been shown that positive clinical effect of rIFN-alpha therapy was associated with a partial restoration of initially decreased IFN-alpha and IFN-gamma production by PBL, and paralleled by increasing the cell IFN-alpha sensitivity.

Dynamics of Interferon System State Parameters during the Immune Therapy of Multiple Sclerosis: Individual Analysis.

Three patients with obvious multiple sclerosis (MS) were observed in a dynamics of immune therapy based on administration of rIFN-beta preparation. The ability to produce IFN-alpha and IFN-gamma by stimulated peripheral blood leukocytes (PBL) of the patients was tested simultaneously with detection of PBL response to the in vitro IFN-beta priming action on the every 10th day of observation. It has been shown that positive clinical effect of recombinant IFN-beta therapy was associated with a partial restoration of initially decreased IFN-beta and IFN-beta production by PBL, and paralleled by reducing the cell IFN-beta sensitivity.