Morphological and Molecular Analysis of Two Mycophagous Nematodes, and (Nematoda: Aphelenchoididae) from Florida Strawberry.

From 2016 to 2021, nematode surveys in Florida strawberry fields revealed several species of foliar nematodes ( spp.). was detected only in 2016 and 2017 on photosynthetic strawberry leaves/buds, but other not well characterized populations of sp.

Recombinase Polymerase Amplification assay for detection of the British root-knot nematode, .

Recombinase polymerase amplification (RPA) is an isothermal nucleic acid amplification technique that has been adopted for simple, robust, rapid, reliable diagnostics of nematodes. In this study, the real-time RPA assay and RPA assay combined with lateral flow dipsticks (LF-RPA) have been developed targeting the ITS rRNA gene of the British root-knot nematode, . The assay provided specific and rapid detection of this root-knot nematode species from crude nematode extracts without a DNA extraction step with a sensitivity of 0.

Rathayibacter tanaceti sp. nov., a Novel Actinobacterium from Tanacetum vulgare Infested by Foliar Nematode Aphelenchoides sp.

Two novel yellow-pigmented, rod-shaped and non-motile coryneform actinobacteria, strains VKM Ac-2596 and VKM Ac-2761, were isolated from a plant Tanacetum vulgare (Asteraceae) infested by foliar nematode Aphelenchoides sp. The strains exhibited the highest 16S rRNA gene sequence similarities to Rathayibacter agropyri CA4 (99.71%), Rathayibacter rathayi DSM 7485 (99.

Phytopathogenic Strains Circulating on Leguminous Plants, Alternative Hosts and Weeds in Russia.

Many bacterial plant pathogens have a broad host range important for their life cycle. Alternate hosts from plant families other than the main (primary) host support the survival and dissemination of the pathogen population even in absence of main host plants. Metabolic peculiarities of main and alternative host plants can affect genetic diversity within and between the pathogen populations isolated from those plants.

Rapid Detection of the Strawberry Foliar Nematode Using Recombinase Polymerase Amplification Assay with Lateral Flow Dipsticks.

Rapid and reliable diagnostic methods for plant-parasitic nematodes are critical for facilitating the selection of effective control measures. A diagnostic recombinase polymerase amplification (RPA) assay for using a TwistAmp Basic Kit (TwistDx, Cambridge, UK) and AmplifyRP Acceler8 Discovery Kit (Agdia, Elkhart, IN, USA) combined with lateral flow dipsticks (LF) has been developed. In this study, a LF-RPA assay was designed that targets the ITS rRNA gene of This assay enables the specific detection of from crude nematode extracts without a DNA extraction step, and from DNA extracts of plant tissues infected with this nematode species.

Correction: Ryss, A.Y.; Subbotin, S.A. New Records of Wood- and Bark-Inhabiting Nematodes from Woody Plants with a Description of sp. n. (Aphelenchoididae: Parasitaphelenchinae) from Russia. 2023, , 382.

In the original publication [...

Morphological and Molecular Diversity among Pin Nematodes of the Genus (Nematoda: Paratylenchidae) from Florida and Other Localities and Molecular Phylogeny of the Genus.

Pin nematodes ( spp.) are root parasites found worldwide. They have different life cycles and feeding habits and can damage a wide range of plants.

Draft Genome Sequences of 11 Rhodopsin Gene-Containing Actinobacteria () from Saline Arid Habitats in the Central Asian Deserts.

Draft genome sequences of 11 strains of putative new species of were generated using Illumina technology. The genome sizes ranged from 4.19 to 4.

Molecular and Morphological Characterization of n. Sp. (Nematoda: Tylenchida) from Corn () in South Carolina.

Specimens of a tylenchid nematode were recovered in 2019 from soil samples collected from a corn field, located in Pickens County, South Carolina, USA. A moderate number of sp. adults (females and males) were recovered.

Distribution of Bacterial Endosymbionts of the Clade in Plant-Parasitic Nematodes.

Bacteria of the genus " Cardinium" and related organisms composing the clade are intracellular endosymbionts frequently occurring in several arthropod groups, freshwater mussels and plant-parasitic nematodes. Phylogenetic analyses based on two gene sequences (16S rRNA and ) showed that the clade comprised at least five groups: A, B, C, D and E. In this study, a screening of 142 samples of plant-parasitic nematodes belonging to 93 species from 12 families and two orders using PCR with specific primers and sequencing, revealed bacteria of clade in 14 nematode samples belonging to 12 species of cyst nematodes of the family Heteroderidae.

New Records of Wood- and Bark-Inhabiting Nematodes from Woody Plants with a Description of sp. n. (Aphelenchoididae: Parasitaphelenchinae) from Russia.

Wood- and bark-inhabiting parasitic nematodes are of great economic importance. Nematodes can cause wilt diseases in conifers and deciduous trees. In 2014-2022, during nematology surveys conducted in different regions of Russia and Belarus, adults and dauer juveniles of nematodes were collected from wood, bark and beetle vectors.

Draft Genome Sequences of 9 Actinobacteria from the Family Associated with Insect- and Nematode-Damaged Plants.

Draft genome sequences of 9 strains of known and putative new species of isolated from insect- and nematode-damaged plants were generated using Illumina technology. The data obtained will contribute to the development of the genome-based prokaryote taxonomy and the knowledge on the biology of the microbial group investigated.

Morphological and Molecular Characterization of Brzeski, 1998 (Nematoda: Heteroderidae) from Virginia, USA.

In August of 2021, several cysts with juveniles and eggs were discovered during a vegetation survey conducted at the Arlington National Cemetery, Virginia. Eight soil samples were collected from the rhizosphere region of the common grass () and processed at the Mycology and Nematology Genetic Diversity and Biology Laboratory (MNGDBL). Cysts were light to dark brown in color, and oval to pear-shaped without bullae in young cysts but present in older cysts and with prominent vulval cone.

Expanding the RNA virome of nematodes and other soil-inhabiting organisms.

In recent years, several newly discovered viruses infecting free-living nematodes, sedentary plant-parasitic nematodes, and migratory root lesion nematodes have been described. However, to the best of our knowledge, no comprehensive research focusing exclusively on metagenomic analysis of the soil nematode community virome has thus far been carried out. In this work, we have attempted to bridge this gap by investigating viral communities that are associated with soil-inhabiting organisms, particularly nematodes.

Recombinase Polymerase Amplification assays for detection of the major tropical root-knot nematodes.

Detection of root-knot nematodes (RKN) in soil and plant samples is crucial to prevent its spread and select effective control measures. In this study, Recombinase Polymerase Amplification (RPA) assays using lateral flow dipsticks (LF-RPA) and real-time fluorescence detection (real-time RPA) were developed to detect the RKN species from tropical complex using a group-specific primer-probe set and using a species-specific primer-probe set. The results of the real time RPA assays in series of crude nematode extracts showed reliable detection within 16 min with a sensitivity of 1/100 of a second-stage juvenile in a reaction tube.

Report of the Parana coffee root-knot nematode, (Tylenchida: Meloidogynidae) from sp. in the continental United States.

In May 2021, the Parana coffee root-knot nematode, was identified using molecular markers from a potted elephant ear plant ( sp.) originated from San Antonio, Texas, USA. This nematode was found in a mixture with the peanut root-knot nematode, The molecular analysis showed that the intergenic -16S gene region and the D2-D3 of 28S rRNA gene sequences allowed differentiating from the related root-knot nematode species of the tropical group.

First report of Seville root-knot nematode, (Nematoda: Meloidogynidae) in the USA and North America.

A high number of second stage juveniles of the root-knot nematode were recovered from soil samples collected from a corn field, located in Pickens County, South Carolina, USA in 2019. Extracted nematodes were examined morphologically and molecularly for species identification which indicated that the specimens of root knot juveniles were The morphological examination and morphometric details from second-stage juveniles were consistent with the original description and redescriptions of this species. The ITS rRNA, D2-D3 expansion segments of 28S rRNA, intergenic -16S region, and gene sequences were obtained from the South Carolina population of .

Molecular and morphological characterization of a first report of Cid del Prado Vera & Subbotin, 2014 (Nematoda: Heteroderidae) from Minnesota, the United States of America.

Cid del Prado Vera & Subbotin, 2014 cysts were discovered during a Pale Potato Cyst Nematode (PCN) survey conducted by Minnesota Department of Agriculture as part of the Animal and Plant Health Inspection Service (APHIS) efforts to survey states for the presence of PCN. The soil samples were collected from a potato field, located in Karlstad, Kittson County, Minnesota, USA. Two out of 175 vials submitted for identification to the Mycology and Nematology Genetic Diversity and Biology Laboratory (MNGDBL) contained few cysts and juveniles of .

Morphological and molecular characterization of n. sp. (Tylenchida: Paratylenchidae) from the rhizosphere of pine tree ( Mill) in Maryland, USA.

The pin nematode, n. sp. collected from rhizosphere soil of a Virginia pine tree ( Mill) growing in Little Paint Branch Park, Beltsville, Prince George's County, Maryland, USA, is described and illustrated along with light and scanning electron photomicrographs.

Report of the Texas peanut root-knot nematode, (Tylenchida: Meloidogynidae) from American pitcher plants ( sp.) in California.

During the winter and spring of 2021, the root-knot nematodes were extracted from samples of galled roots of potted American pitcher plants ( sp.). Samples were collected from a botanical garden nursery in Los Angeles County, California.

' Xiphinematincola pachtaicus' gen. nov., sp. nov., an endosymbiotic bacterium associated with nematode species of the genus (Nematoda, Longidoridae).

An intracellular bacterium, strain IAS, was observed to infect several species of the plant-parasitic nematode genus (, , , , and ). The bacterium could not be recovered on axenic medium. The 16S rRNA gene sequence of IAS was found to be new, being related to the family Burkholderiaceae, class Betaproteobacteria.

Morphological and molecular characterization of Chitwood, 1957 (Dorylaimida: Leptonchidae) from Florida, with the first molecular study of the genus.

A population of , recently collected under the canopy of associated live oak trees in north Florida, was studied and described with an integrative approach, including the first molecular study of the genus. This Florida population is characterized by its 2.30 to 3.

Morphology, development stages, and phylogeny of the (Nematoda: Diplogastridae), a phoront of the bark beetle from the elm Huds. in Northwest Russia.

The nematode was found in galleries, adults, and larvae of , the vector of the Dutch elm disease, in St. Petersburg parks. This nematode co-occurred with , which is another phoretic partner of .

First report of the bent seed gall nematode, (Steinbuch, 1799) Filipjev, 1936 from L. in Wyoming, USA.

In September 2020, several plants of fowl bluegrass, with seed galls were collected on a bank of river in Teton County, Wyoming, USA. Isolated nematodes were identified by both morphological and molecular methods as This is a first report of in Wyoming and its report on fowl bluegrass.

On the molecular identity of (Nematoda: Tylenchida).

In this study, molecular characterization of collected from the type locality in Four Mile Run, Fall Church, Virginia using D2-D3 of 28 S rRNA and ITS rRNA gene sequences was provided. We molecularly also characterized, specimens collected from grasses in Devils Lake, Ramsey County, North Dakota indicated as the type locality in the original description of by Cobb (1923). These nematodes were identified as representatives of the species Populations of belonging to the molecular types A and B, and previously designated by Van den Berg et al.