[Effectiveness and safety of biological therapy in patients with severe asthma in a real clinical practice].

Aim: To assess effectiveness and safety of biological therapy in patients with severe asthma during 5 yr follow-up.

Materials And Methods: We recruited 129 adult outpatients (29% males) aged 18-81 yrs with severe asthma were followed up during 5 yrs and were examined for every 3-6 months. Eighty five patients were treated by conventional therapy (ICS/LABA ± tiotropium, montelukast, OCS) only and 44 pts additionally received biologicals (оmalizumab - 9 pts, мepolizumab - 8 pts, benralizumab - 11 pts, dupilumab - 16 pts).

Markers of eosinophilic airway inflammation in patients with asthma and allergic rhinitis.

Measurement of airway inflammation is an important step to determine phenotype of asthma and allergic rhinitis (AR). To assess the level of nitric oxide in exhaled air (FeNO), nasal fraction of nitric oxide (nasal NO), their relationship with clinical control and blood eosinophils in patients with steroid-naive mild and moderate asthma and AR. One hundred forty-seven patients (65 men), ages 26-49.

ERS statement: a core outcome set for clinical trials evaluating the management of COPD exacerbations.

Clinical trials evaluating the management of acute exacerbations of COPD assess heterogeneous outcomes, often omitting those that are clinically relevant or more important to patients. We have developed a core outcome set, a consensus-based minimum set of important outcomes that we recommend are evaluated in all future clinical trials on exacerbations management, to improve their quality and comparability. COPD exacerbations outcomes were identified through methodological systematic reviews and qualitative interviews with 86 patients from 11 countries globally.

[Development of surgery of the heart and great vessels in Rassia during the first half of the 20th century].

Analysed in the article are scientific priorities of Russian cardiovascular surgeons in the first half of the 20th century, showing rapid and successful development of surgery of the lungs, heart, and great vessels over a comparatively short time period, also underlining the fundamental works of Yu.Yu. Dzhanelidze, S.

Core outcome set for the management of acute exacerbations of chronic obstructive pulmonary disease: the COS-AECOPD ERS Task Force study protocol.

Randomised controlled trials (RCTs) on the management of COPD exacerbations evaluate heterogeneous outcomes, often omitting those that are clinically important and patient relevant. This limits their usability and comparability. A core outcome set (COS) is a consensus-based minimum set of clinically important outcomes that should be evaluated in all RCTs in specific areas of health care.

[Severe asthma: Characteristics of patients in clinical practice].

Aim: To provide clinical characteristics of severe asthma (SA) patients encountered in clinical practice.

Subjects And Methods: A cross-sectional cohort study was performed to cover 119 outpatients aged 22-82 years. SA was diagnosed according to the ERS/ATS criteria (2014).

Methylation-based analysis of circulating DNA for breast tumor screening.

The applicability of cyclin D2 and RARbeta2 methylated markers for the development of a breast tumor screening assay was evaluated. Overall, 76 volunteers (mean age, 50.4 years), including clinically healthy women and women with breast lesions, were enrolled in a blind study of methylation of the cyclin D2 and RARbeta2 genes.

[Prevalence of bronchial asthma and allergic rhinitis in the adult population of St. Petersburg].

Aim: To evaluate prevalence of bronchial asthma (BA) and allergic rhinitis (AR) among adult population of St-Petersburg.

Material And Methods: Responders to ECRHS and B. Sibbald & E.

[DNA-containing polygonal structures detected in somatic nuclei of Bursaria ovata during preparation to cryptobiosis].

Supramolecular chromatin organization of the somatic nucleus (macronucleus--Ma) was studied in a free-living unicellular eukaryotic organism, the ciliate Bursaria ovata Beers 1952, at two late successive stages of its encystment (in the state of preparation to cryptobiosis). A modified Miller's method (Sergejeva et al., 1987) and the same technique in combination with high resolution DNA autoradiography were used.

[Effectiveness of detection of bronchial asthma with two international questionnaires].

Aim: Assessment of two questionnaires (ECRHS and IUATLD) for efficiency in bronchial asthma (BA) detection.

Material And Methods: 196 patients aged 16-59 years seeking advice of a doctor for pulmonary or extrapulmonary complaints. All of them have not been previously examined for BA.

[Diagnostic value of a novel nutrient medium for isolation and cultivation of pathogens causing enteric yersiniosis and pseudotuberculosis].

A new nutrient medium for isolation and cultivation of the causative agents of enteric yersiniosis and pseudotuberculosis was found to have advantages over Endo medium in its differentiating and inhibiting properties. This medium permitted the easy differentiation of Yersinia pseudotuberculosis from Y. enterocolitica, as well as from Escherichia coli, Shigella flexneri, Klebsiella pneumoniae, K.

[The methods of traditional medicine in outpatient polyclinic practice].

Some 7950 patients have been treated at the traditional medicine department of the Consulting and Diagnostic Center N52, 2/3rds of them came after long and unsuccessful medicamental treatment. Psychotherapy, manual therapy and acupuncture-reflex methods were successful in 86-93% of cases. They are recommended for local clinics provided that the latter are properly equipped and stuffed.

[Biomechanical aspects of the process of formation of food vacuoles in the infusoria Bursaria truncatella under changed gravity].

Results from clinostatic and centrifugal laboratory experiments in which there has been evaluated an activity of digestive process in infusoria Bursaria truncatella by the content of vacuole numbers and its change in the cell under changed gravity are presented. It is indicated that the extended clinostatic exposure of infusoria stimulates their digestive activity and an increased gravity (centrifugation 2 g, 5 g) inhibits this process. In these examinations, an effort was made to explain the obtained results started from the propositions of cell biomechanics and bioenergetics.

[Effects of altered gravity on the culture of unicellular eucaryotic organisms, Bursaria truncatella (Ciliophora)].

The paper reports the results of experiments with centrifugation and clinostating. Growth rate, cellular division and several morphofunctional characteristics of unicellular organisms of infusoria Bursaria truncatella in culture were studied under normal (1 g), elevated (hypergravity at 2 and 5 g), and compensated gravity. The data point to certain changes in the functional activity and morphology of cells consequent to long-time cultivation under these conditions.

[Functional state of blood vessels of the skin--a criterion after exposure to laser irradiation].

The status of the microcirculatory skin channel under laser irradiation with wave length 10.6 mkm of different energetic exposures has been studied. Higher density of vessels per unit of area, longer diameter of the vessels, disturbances in the blood flow have been observed in the dynamics of exposure, which can be used as a criterion of harmful skin effect of irradiation.

[Data for the assessment of maximum allowable concentration of dust in the air of work zone during production of permanent magnets based on samarium and cobalt].

The hygienic studies revealed that one of the most unfavourable factors in permanent magnet production is presented by the composite dust of cobalt and samarium. The toxico-hygienic assessment of the composite determined its MAC in the working zone air at the level 0.05 mg/m3 (as to cobalt).

[Sensitivity of animals to CO2 laser radiation].

The study was undertaken to analyze the state of visual, cardiovascular and antioxidant systems along with peripheral blood exposed to laser emission with the wavelength of 10.6 mm and energetic exposure equal to that of threshold (21 J.cm2) and subthreshold (1/7 of the threshold value).

[Working conditions in manufacturing stable magnets using the powder metallurgy method].

The data are presented on the conditions and the character of work when manufacturing samarium- and cobalt-based permanent magnets. Major unfavourable factors of samarium/cobalt-containing dusts are stressed, the chemical and disperse dust composition being described. Highlighted is the necessity of developing a set of preventive measures.

[Experimental study of the immunological activity of a preparation made from the antigenic complexes of opportunistic microorganisms administered by different routes].

The data obtained in the experimental study of the humoral factors of local and systemic immunity, as well as the morphofunctional changes of internal organs, after multiple subcutaneous and aerosol immunization with the combined preparation of Proteus, Staphylococcus, Klebsiella pneumoniae and Escherichia coli antigens (preparation nC-4) are presented. The subcutaneous and aerosol administration of preparation nC-4 has been found to induce an increase in the levels of antibodies to all components of the preparation in the blood serum and in respiratory tract secretions. The introduction of the preparation through the respiratory tract resulted in an earlier intensive accumulation of specific antibodies and IgA in respiratory tract secretions.

[Characteristics of the immune response to a single peroral administration of different doses of corpuscular pertussis vaccine].

The effect of oral administrations of different doses of pertussis vaccine on the humoral and cell-mediated responses of systemic immunity and on the immunomorphological transformation of the mucous membrane of the small intestine was studied in (CBA X C57BL/6)F1 mice. On day 28 after the administration of all the tested doses of pertussis vaccine the animals were found to have a high degree of protection from the development of meningoencephalitis induced by the inoculation of Bordetella pertussis in the absence of specific hemagglutinins in their blood sera. At the same time the formation of spontaneous and immune rosette-forming cells and splenocytes was found to be inversely related to the administered dose.

[Structure of the macronucleus chromatin of the ciliate Bursaria truncatella. III. Chromatin organization in resting cysts and during excysting].

Structural organization of macronuclear chromatin of a ciliate Bursaria truncatella was studied electronmicroscopically by means of Miller's technique and negative staining of resting cysts and at excysting. In resting cysts practically all the macronuclear chromatin was shown to be organized into compact chromatin clumps 100-300 nm in size. At excysting a natural decompactization of the chromatin clumps occurred and radial loop-shaped chromatin fibres appeared around the clumps.