Dataset on investigating nucleation and growth kinetics of methane hydrate in aqueous methanol solutions.

This work systematically investigates the effect of methanol (MeOH) in a wide range of concentrations (0, 1, 2.5, 5, 10, 20, 30, 40, and 50 mass%) on methane hydrate nucleation and growth kinetics. Multiple measurements of gas hydrate onset temperatures and pressures for CH-HO and CH-MeOH-HO systems were performed by ramp cooling experiments (1 K/h) using sapphire rocking cell RCS6 apparatus.

Data on searching for synergy between alcohol and salt to produce more potent and environmentally benign gas hydrate inhibitors.

In order to systematically study the synergistic effect of gas hydrate inhibition with mixtures of methanol (MeOH) and magnesium chloride (MgCl), the impact of these compounds on the thermodynamic stability of methane hydrate in the systems of CH-MeOH-HO, CH-MgCl-HO, and CH-MeOH-MgCl-HO was experimentally investigated. The pressure and temperature conditions of the three-phase vapor-aqueous solution-gas hydrate equilibrium were determined for these systems. The resulting dataset has 164 equilibrium points within the range of 234-289 K and 3-13 MPa.

Dataset for the dimethyl sulfoxide as a novel thermodynamic inhibitor of carbon dioxide hydrate formation.

The temperatures and pressures of the three-phase equilibrium V-L-H (gas - aqueous solution - gas hydrate) were measured in the CO - HO - dimethyl sulfoxide (DMSO) system at concentrations of organic solute in the aqueous phase up to 50 mass%. Measurements of CO hydrate equilibrium conditions were carried out using a constant volume autoclave by continuous heating at a rate of 0.1 K/h with simultaneous stirring of fluids by a four-blade agitator at 600 rpm.

THz Smith-Purcell and grating transition radiation from metasurface: experiment and theory.

We report the results of experimental and theoretical studies of monochromatic coherent terahertz radiation generated by a short relativistic electron bunch interacting with a metasurface. The metasurface consists of subwavelength metal elements arranged on a dielectric substrate. The constructed theory explains the experimental spectra of Smith-Purcell radiation and grating transition radiation with very high precision.

Multicopy Targeted Integration for Accelerated Development of High-Producing Chinese Hamster Ovary Cells.

The ever-growing biopharmaceutical industry relies on the production of recombinant therapeutic proteins in Chinese hamster ovary (CHO) cells. The traditional timelines of CHO cell line development can be significantly shortened by the use of targeted gene integration (TI). However, broad use of TI has been limited due to the low specific productivity () of TI-generated clones.

CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cells.

The emergence of CRISPR/Cas9 system as a precise and affordable method for genome editing has prompted its rapid adoption for the targeted integration of transgenes in Chinese hamster ovary (CHO) cells. Targeted gene integration allows the generation of stable cell lines with a controlled and predictable behavior, which is an important feature for the rational design of cell factories aimed at the large-scale production of recombinant proteins. Here we present the protocol for CRISPR/Cas9-mediated integration of a gene expression cassette into a specific genomic locus in CHO cells using homology-directed DNA repair.

Minimizing Clonal Variation during Mammalian Cell Line Engineering for Improved Systems Biology Data Generation.

Mammalian cells are widely used to express genes for basic biology studies and biopharmaceuticals. Current methods for generation of engineered cell lines introduce high genomic and phenotypic diversity, which hamper studies of gene functions and discovery of novel cellular mechanisms. Here, we minimized clonal variation by integrating a landing pad for recombinase-mediated cassette exchange site-specifically into the genome of CHO cells using CRISPR and generated subclones expressing four different recombinant proteins.

Using Titer and Titer Normalized to Confluence Are Complementary Strategies for Obtaining Chinese Hamster Ovary Cell Lines with High Volumetric Productivity of Etanercept.

The selection of clonally derived Chinese hamster ovary (CHO) cell lines with the highest production rate of recombinant glycoproteins remains a big challenge during early stages of cell line development. Different strategies using either product titer or product titer normalized to cell number are being used to assess suspension-adapted clones when grown statically in microtiter plates. However, no reported study so far has performed a direct head-to-head comparison of these two early reporters for predicting clone performance.

Smith-Purcell radiation from periodic beams.

Smith-Purcell effect is well known as a source of monochromatic electromagnetic radiation. In this paper we present the generalized theory of Smith-Purcell radiation from periodic beams. The form-factors describing both coherent and incoherent regimes of radiation are calculated.

[Mercury content in fish].

Studied was the content of mercury in certain fish species from ocean and the Black Sea catch (mackerel, scad, and merluccius), sold on the home markets. The study of samples of merluccius revealed that the average level of mercury was 0.088 ppm, the deviations being from 0.

[Determination of the concentration of copper, lead, cadmium and zinc in milk by atomic absorption spectrophotometry].

Developed was an atom-absorption method for determining microconcentrations of copper, lead, cadmium, and zink in milk originating from various regions of this country. The study was carried out by means of an atom-absorption spectrophotometer AA-5. Established were the optimal conditions for determining the trace elements reaching the following values of susceptibility: for copper--0.

[Thin-layer chromatographic demonstration of the herbicide zenkor].

Established were the thin-layer chromatography conditions for the demonstration of the herbicide Zenkor as a pure substance and a preparation. Comparative experiments were carried out applying Thomson and Stanley's thin-layer chromatography conditions and the authors' personal ones. Most adequate proved to be the following conditions: sorbent-Kieselgel 60F 254 (Merck); solvent -- chloroform: 1,4-dioxan (9:1); developer -- ultraviolet light (authors' technique).