Ozone impacts on vegetation in a nitrogen enriched and changing climate.

This paper provides a process-oriented perspective on the combined effects of ozone (O3), climate change and/or nitrogen (N) on vegetation. Whereas increasing CO2 in controlled environments or open-top chambers often ameliorates effects of O3 on leaf physiology, growth and C allocation, this is less likely in the field. Combined responses to elevated temperature and O3 have rarely been studied even though some critical growth stages such as seed initiation are sensitive to both.

Stomata are less responsive to environmental stimuli in high background ozone in Dactylis glomerata and Ranunculus acris.

Two mesotrophic grassland species, Ranunculus acris and Dactylis glomerata were exposed to a range of ozone treatments (16.2-89.5 ppb 24 h mean) and two watering regimes under naturally fluctuating photosynthetically active radiation (PAR), vapour pressure deficit (VPD) and temperature.

Reduced soil water availability did not protect two competing grassland species from the negative effects of increasing background ozone.

Two common (semi-) natural temperate grassland species, Dactylis glomerata and Ranunculus acris, were grown in competition and exposed to two watering regimes: well-watered (WW, 20-40% v/v) and reduced-watered (RW, 7.5-20% v/v) in combination with eight ozone treatments ranging from pre-industrial to predicted 2100 background levels. For both species there was a significant increase in leaf damage with increasing background ozone concentration.