Publications by authors named "Serena Ngiimei D"
Article Synopsis
- - Calcium (Ca) is essential for cellular processes and must be carefully regulated, making it important to measure its levels in cells, particularly in filamentous fungi.
- - Various techniques exist for measuring Ca concentrations, including the use of calcium-sensitive fluorescent chlortetracycline (CTC), Ca-selective microelectrodes, radioactive Ca isotopes, and genetically engineered calcium indicators (GECIs) like aequorins and RGECOs.
- - Each method has its own advantages and limitations, with CTC fluorescence useful for visualization, microelectrodes for detecting Ca flux, and radioactive tracers for quantifying Ca in cellular compartments.
The cation diffusion facilitator (CDF) family of zinc transporters plays a crucial role in zinc homeostasis in eukaryotes, including fungi. Here, we investigated the cell functions and genetic interactions of CDF zinc transporters -1 and -2 in . The Δ-1 mutant could not grow in a high-zinc environment, indicating that the zinc transporter protein ZRC-1 was essential for growth in high-zinc conditions.
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