Publications by authors named "Serena Cornelius"
Article Synopsis
- Influenza A viruses contain eight genomic RNAs that are transcribed in the host cell nucleus, with two undergoing alternative splicing to produce M1 and M2 mRNAs.
- The NS1-BP protein helps facilitate the splicing of M1 to M2 mRNA by competing with the viral NS1 protein, which normally inhibits mRNA export.
- The research suggests a mechanism where spliced M mRNAs are exported from the nucleus despite NS1's inhibitory effects, crucial for the production of viral proteins needed for replication and virus release.
Organoids are a reliable method for modeling organ tissue due to their self-organizing properties and retention of function and architecture after propagation from primary tissue or stem cells. This method of organoid generation forgoes single-cell differentiation through multiple passages and instead uses differential centrifugation to isolate mammary epithelial organoids from mechanically and enzymatically dissociated tissues. This protocol provides a streamlined technique for rapidly producing small and large epithelial organoids from both mouse and human mammary tissue in addition to techniques for organoid embedding in collagen and basement extracellular matrix.
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