Cytoplasmic Accumulation and Permeability of Antibiotics in Gram Positive and Gram Negative Bacteria Visualized in Real-Time via a Fluorogenic Tagging Strategy.

In this study, we describe the first real-time live cell assay for compound accumulation and permeability in both Gram positive and Gram negative bacteria. The assay utilizes a novel fluorogenic tagging strategy that permits direct visualization of compound accumulation dynamics in the cytoplasm of live cells, unobscured by washing or other processing steps. Quantitative differences could be reproducibly measured by flow cytometry at compound concentrations below the limit of detection for MS-based approaches.

Toward Combined Carbon Capture and Recycling: Addition of an Amine Alters Product Selectivity from CO to Formic Acid in Manganese Catalyzed Reduction of CO.

Owing to the energetic cost associated with CO release in carbon capture (CC), the combination of carbon capture and recycling (CCR) is an emerging area of research. In this approach, "captured CO," typically generated by addition of amines, serves as a substrate for subsequent reduction. Herein, we report that the reduction of CO in the presence of morpholine (generating mixtures of the corresponding carbamate and carbamic acid) with a well-established Mn electrocatalyst changes the product selectivity from CO to H and formate.