Forage conservation is a neglected nitrous oxide source.

Agricultural activities are the major anthropogenic source of nitrous oxide ( ), an important greenhouse gas and ozone-depleting substance. However, the role of forage conservation as a potential source of has rarely been studied. We investigated production from the simulated silage of the three major crops-maize, alfalfa, and sorghum-used for silage in the United States, which comprises over 90% of the total silage production.

The relative contributions of Mn(III) and Mn(IV) in manganese dioxide polymorphs to bisphenol A degradation.

Polymorphs of MnO comprise Mn(III) and Mn(IV), which are both strong oxidants capable of BPA degradation, but their relative contributions are unclear. To advance process understanding, the reactivities of biogenic MnO prepared using Roseobacter sp. AzwK-3b and synthetic MnO (i.

Evaluation and optimization of lysis method for microbial DNA extraction from epiphytic phyllosphere samples.

Analysis of microbial communities in the epiphytic phyllosphere can be challenging, especially when applying sequencing-based techniques, owing to the interference of plant-derived biomolecules such as nucleic acids. A review of recent studies on the epiphytic microbiome revealed that both mechanical and enzymatic lysis methods are widely used. Here, we evaluated the effects of the two lysis methods on DNA extraction yield, purity, integrity, and microbial 16S rRNA gene copy number per ng of template genomic DNA under different extraction conditions.