Efficient Plant Regeneration System from Leaf Explant Cultures of via Somatic Embryogenesis.

This study aimed to establish an efficient plant regeneration system from leaf-derived embryogenic structure cultures of . To induce embryogenic structures, fully expanded leaf explants of were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.1, 0.

Efficient plant regeneration from embryogenic cell suspension cultures of Euonymus alatus.

To establish an efficient plant regeneration system from cell suspension cultures of Euonymus alatus, embryogenic callus formation from immature embryos was investigated. The highest frequency of embryogenic callus formation reached 50% when the immature zygotic embryos were incubated on Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D). At higher concentrations of 2,4-D (over 2 mg/L), the frequency of embryogenic callus formation declined significantly.

An Alcohol Dehydrogenase Gene from sp. Confers Salt Tolerance in Transgenic Tobacco.

salt-responsive gene 1 () was engineered for expression in higher plants, and gene construction was stably incorporated into tobacco plants. We investigated the role of Sysr1 [a member of the alcohol dehydrogenase (ADH) superfamily] by examining the salt tolerance of -overexpressing (-OX) tobacco plants using quantitative real-time polymerase chain reactions, gas chromatography-mass spectrometry, and bioassays. The -OX plants exhibited considerably increased ADH activity and tolerance to salt stress conditions.