Identification of two AFLP markers linked to bacterial wilt resistance in tomato and conversion to SCAR markers.

Tomato bacterial wilt (BW) incited by Ralstonia solanacearum is a constraint on tomato production in tropical, subtropical and humid regions of the world. In this paper, we present the results of a research aimed at the identification of PCR-based markers amplified fragment length polymorphism (AFLP) linked to the genes that confer resistance to tomato BW. To this purpose, bulked segregant analysis was applied to an F(2) population segregating for the BW resistant gene and derived from the pair-cross between a BW resistant cultivar T51A and the susceptible cultivar T9230.

[Identification of AFLP markers linked to bacterial wilt resistance gene in tomato].

A cross between bacterial wilt resistant tomato variety "T51A"and susceptible variety 'T9230' was made for mapping bacterial wilt resistance gene(s). Through inoculation test of its F1 and F2 progeny, it was proved that the resistance of 'T51A' to bacterial wilt was controlled by one heterozygous gene and cytoplasm. With 64 EcoR I/Muse I primer combinations, AFLP analysis was performed on two parents and their F2 resistant and susceptible bulks.

[Primary targeting of functional regions involved in transcriptional regulation on watermelon fruit-specific promoter WSP].

Fruit ripening is associated with a number of physiological and biochemical changes. They include degradation of chlorophyll, synthesis of flavor compounds, carotenoid biosynthesis, conversion of starch to sugars, cell wall solublisation and fruit softening. These changes are brought about by the expression of specific genes.