Publications by authors named "Senthil-Kumar Devan"
Deciphering the RNA-binding protein network during endosomal mRNA transport.
Proc Natl Acad Sci U S A
November 2024
Article Synopsis
- Microtubule-dependent endosomal transport is essential for distributing cellular components like proteins and mRNAs, but the link between mRNAs and the endosomal surface is not fully understood.
- The research focuses on the RNA-binding protein Rrm4 and reveals a novel form of the MademoiseLLE (MLLE) domain, characterized by a unique seven-helical bundle that enhances its binding capability.
- The study also compares this new MLLE domain with the canonical one from poly(A)-binding protein Pab1, uncovering important structural differences that help predict and verify interactions with other proteins, including human MLLE domains like PABPC1 and UBR5, which aid mRNA attachment during transport.
Spatiotemporal expression can be achieved by transport and translation of mRNAs at defined subcellular sites. An emerging mechanism mediating mRNA trafficking is microtubule-dependent co-transport on shuttling endosomes. Although progress has been made in identifying various components of the endosomal mRNA transport machinery, a mechanistic understanding of how these RNA-binding proteins are connected to endosomes is still lacking.
View Article and Find Full Text PDFIn eukaryotic cells, proteins are targeted to their final subcellular locations with precise timing. A key underlying mechanism is the active transport of cognate mRNAs, which in many systems can be linked intimately to membrane trafficking. A prominent example is the long-distance endosomal transport of mRNAs and their local translation.
View Article and Find Full Text PDFmRNA transport determines spatiotemporal protein expression. Transport units are higher-order ribonucleoprotein complexes containing cargo mRNAs, RNA-binding proteins and accessory proteins. Endosomal mRNA transport in fungal hyphae belongs to the best-studied translocation mechanisms.
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