Global Protein Interactome Mapping in Rice Using Barcode-Indexed PCR Coupled with HiFi Long-Read Sequencing.

Establishing the protein-protein interaction network sheds light on functional genomics studies by providing insights from known counterparts. However, the rice interactome has barely been studied due to the lack of massive, reliable, and cost-effective methodologies. Here, the development of a barcode-indexed PCR coupled with HiFi long-read sequencing pipeline (BIP-seq) is reported for high throughput Protein Protein Interaction (PPI)identification.

Evaluation and analysis of the transmission route of sigmoid sinus tinnitus by HRCT of the temporal bone: A retrospective analysis.

Objective: This study aims to analyze anatomical parameters of the transmission route of sigmoid sinus tinnitus (SST) to explore its mechanism and speculate on possible responsible anatomical abnormalities.

Methods: Clinical data were retrospectively collected from SST and sigmoid sinus wall dehiscence (SSWD) patients suggested by temporal bone high resolution computed tomography (HRCT), with and without tinnitus, at the First Affiliated Hospital of Chongqing Medical University from January 2015 to August 2022. Patients were divided into SSWD tinnitus ( = 61), and non-tinnitus ( = 60) groups based on HRCT features.

Analysis of factors influencing the awareness of inpatients regarding hospital clinical pharmacy services and willingness to pay: a multicenter survey in Hebei Province, China.

Introduction: Pharmacists are increasingly adopting patient-centered roles, improving healthcare outcomes by reducing medication errors and costs. In China, recent healthcare reforms recognize and compensate for pharmacy services. However, patient awareness of these services and their willingness to pay (WTP) remain underexplored.

Cost-effectiveness analysis of pembrolizumab plus chemotherapy versus chemotherapy in untreated advanced pleural mesothelioma in the Chinese healthcare system.

Objective: The combination of pembrolizumab and chemotherapy has demonstrated notable clinical advantages in improving overall survival than chemotherapy alone for patients with untreated advanced pleural mesothelioma. The purpose of this study was to assess its cost-effectiveness.

Materials And Methods: A Markov state-transition model was constructed using data from the IND227 phase 3 randomized clinical trial.

Bridging brain and blood: a prospective view on neuroimaging-exosome correlations in HIV-associated neurocognitive disorders.

HIV-associated neurocognitive disorder (HAND) is a complex neurological complication resulting from human immunodeficiency virus (HIV) infection, affecting about 50% of individuals with HIV and significantly diminishing their quality of life. HAND includes a variety of cognitive, motor, and behavioral disorders, severely impacting patients' quality of life and social functioning. Although combination antiretroviral therapy (cART) has greatly improved the prognosis for HIV patients, the incidence of HAND remains high, underscoring the urgent need to better understand its pathological mechanisms and develop early diagnostic methods.

Efficacy of a hydrogen-oxygen generator in treating cigarette smoke-induced chronic obstructive pulmonary disease in rats.

Current treatments for chronic obstructive pulmonary disease (COPD), a common respiratory condition, include oxygen therapy and steroids for temporary relief. In this study, we established a rat model of cigarette smoke (CS)-induced COPD and investigated the benefits of a hydrogen-oxygen generator in this model. CS-exposed rats were treated using either a hydrogen-oxygen generator or a steroid.

Efficacy of microwave ablation for intrahepatic cholangiocarcinoma: a systematic review and meta-analysis.

Background: Data on overall survival (OS) and progression-free survival (PFS) after microwave ablation (MWA) for intrahepatic cholangiocarcinoma (ICC) are scarce. We conducted a systematic review of the safety and efficacy of MWA for ICC.

Methods: The PubMed, Embase, Web of Science, and Cochrane Library databases were searched for studies reporting the outcomes of MWA for ICC.

Racial and sexual differences of eyebrow and eyelid morphology: three-dimensional analysis in young Caucasian and Chinese populations.

Background: With globalization, oculoplastic surgeons must understand the intricate morphological nuances of the periocular region across ethnicities to ensure precise treatment and avoid facial disharmony or dysfunction. Direct comparisons in two-dimensional (2D)-based periocular morphology between studies can be challenging due to the limited number of parameters and complicated variations in equipment, environments, measurement personnel, and methods. Therefore, it is imperative to explore the detailed three-dimensional (3D) periocular morphological disparities between young Caucasian and Chinese populations.

Evaluation of gray-matter and white-matter microstructural abnormalities in children with growth hormone deficiency: a comprehensive assessment with synthetic magnetic resonance imaging.

Background: Pediatric growth hormone deficiency (GHD) is a disease resulting from the impaired growth hormone-insulin-like growth factor-1 (GH-IGF-1) axis, but the effects of GHD on children's behavior and brain microstructural structure alterations have not yet been fully clarified. We aimed to investigate the quantitative profiles of gray matter and white matter in pediatric GHD using synthetic magnetic resonance imaging (MRI).

Methods: The data of 50 children with GHD and 50 typically developing (TD) children were prospectively collected.

Alterations in local activity and whole-brain functional connectivity in human immunodeficiency virus-associated neurocognitive disorders: a resting-state functional magnetic resonance imaging study.

Background: Approximately half of human immunodeficiency virus (HIV) patients experience HIV-associated neurocognitive disorders (HAND); however, the neurophysiological mechanisms underlying HAND remain unclear. This study aimed to evaluate changes in functional brain activity patterns during the early stages of HIV infection by comparing local and global indicators using resting-state functional magnetic resonance imaging (rs-fMRI).

Methods: A total of 165 people living with HIV (PLWH) but without neurocognitive disorders (PWND), 173 patients with asymptomatic neurocognitive impairment (ANI), and 100 matched healthy controls (HCs) were included in the study.

Efficacy and safety of non-focused low-intensity ultrasound technology for subcutaneous lipolysis in the lower abdomen: a clinical study.

Background: With rising demand for non-invasive body contouring, this study aimed to evaluate the efficacy and safety of non-focused, low-intensity ultrasound for lower abdominal lipolysis.

Methods: The enrolled subjects prospectively received 6 weekly sessions of well-setting ultrasound treatment with frequency of 1 MHz and an intensity of 2.2 W/cm2, to target the subcutaneous fat of lower abdomen with three regions (left, middle, and right).

Bacteria Flagella-Mimicking Polymer Multilayer Magnetic Microrobots.

Mass production of biomedical microrobots demands expensive and complex preparation techniques and versatile biocompatible materials. Learning from natural bacteria flagella, the study demonstrates a magnetic polymer multilayer cylindrical microrobot that bestows the controllable propulsion upon an external rotating magnetic field with uniform intensity. The magnetic microrobots are constructed by template-assisted layer-by-layer technique and subsequent functionalization of magnetic particles onto the large opening of the microrobots.

TREM2 promotes the formation of a tumor-supportive microenvironment in hepatocellular carcinoma.

Background: Triggering receptor expressed on myeloid cells 2 (TREM2), a surface receptor predominantly expressed on myeloid cells, is a major hub gene in pathology-induced immune signaling. However, its function in hepatocellular carcinoma (HCC) remains controversial. This study aimed to evaluate the role of TREM2 in the tumor microenvironment in the context of HCC progression.

Multi-gene precision editing tool using CRISPR-Cas12a/Cpf1 system in Ogataea polymorpha.

Background: Ogataea polymorpha, a non-conventional methylotrophic yeast, has demonstrated significant potential for heterologous protein expression and the production of high-value chemicals and biopharmaceuticals. However, the lack of precise and efficient genome editing tools severely hinders the construction of cell factories. Although the CARISP-Cas9 system has been established in Ogataea polymorpha, the gene editing efficiency, especially for multiple genes edition, needs to be further improved.

Endothelial cells under disturbed flow release extracellular vesicles to promote inflammatory polarization of macrophages and accelerate atherosclerosis.

Background: Extracellular vesicles (EVs) derived from endothelial cells (ECs) are increasingly recognized for their role in the initiation and progression of atherosclerosis. ECs experience varying degrees and types of blood flow depending on their specific arterial locations. In regions of disturbed flow, which are predominant sites for atherosclerotic plaque formation, the impact of disturbed flow on the secretion and function of ECs-derived EVs remains unclear.

A new pathogen pattern of acute respiratory tract infections in primary care after COVID-19 pandemic: a multi-center study in southern China.

Background: After the coronavirus disease 2019 (COVID-19) pandemic, no studies on bacterial and atypical pathogens were conducted in primary care. We aimed to describe the etiological composition of acute respiratory tract infections (ARTIs) presenting to primary care with limited resources after the pandemic.

Methods: 1958 adult patients with ARTIs from 17 primary care clinics were recruited prospectively from January 2024 to March 2024.

Deep learning-based CT radiomics predicts prognosis of unresectable hepatocellular carcinoma treated with TACE-HAIC combined with PD-1 inhibitors and tyrosine kinase inhibitors.

Objective: To develop and validate a computed tomography (CT)-based deep learning radiomics model to predict treatment response and progression-free survival (PFS) in patients with unresectable hepatocellular carcinoma (uHCC) treated with transarterial chemoembolization (TACE)-hepatic arterial infusion chemotherapy (HAIC) combined with PD-1 inhibitors and tyrosine kinase inhibitors (TKIs).

Methods: This retrospective study included 172 patients with uHCC who underwent combination therapy of TACE-HAIC with TKIs and PD-1 inhibitors. Among them, 122 were from the Interventional Department of the Harbin Medical University Cancer Hospital, with 92 randomly assigned to the training cohort and 30 cases randomly assigned to the testing cohort.

Dual inhibition of AChE and MAO-B in Alzheimer's disease: machine learning approaches and model interpretations.

Alzheimer's disease (AD) is one of the most prevalent neurodegenerative diseases. Given the multifactorial pathophysiology of AD, monotargeted agents can only alleviate symptoms but not cure AD. Acetylcholinesterase (AChE) and Monoamine oxidase B (MAO-B) are two key targets in the treatment of AD, molecules that inhibiting both targets are considered promising avenue to develop more effective AD therapies.

Exploring and validating associations between six systemic inflammatory indices and ischemic stroke in a middle-aged and old Chinese population.

Background: Inflammation and maladaptive immune mechanisms have been substantiated as integral components in the critical pathological processes of the injury cascade in ischemic stroke (IS). This study aimed to explore the associations between six systemic inflammatory indices and IS in a Chinese population.

Methods: This was a case-control study based on the retrospective review of electronic medical records from two hospitals in Shandong Province, China.

The value of increasing sequencing depth for noninvasive prenatal screening for whole chromosomal aneuploidy.

To evaluate the value of increasing sequencing depths of non-invasive prenatal testing (NIPT) for fetal chromosomal aneuploidies based on the semiconductor sequencing platform. This study recruited a cohort of 59,800 singleton pregnancies from Guangdong Women and Children Hospital between January 2015 and December 2020, including 48,018 cases of NIPT and 11,782 cases of expanded NIPT. Cell-free DNA from plasma samples was sequenced at a sequencing depth of 0.

The necroptosis-related lncRNA ENSG00000253385.1 promotes the progression of esophageal squamous cell carcinoma by targeting the miR-16-2-3p/VDAC1 axis.

Esophageal squamous cell carcinoma (ESCC) is one of the most common digestive malignancies. Our previous studies revealed necroptosis-related lncRNA ENSG00000253385.1 was an independent prognostic factor for ESCC.

Association of pregnancy diet with metabolic adverse outcomes in pregnant women and their children: A Systematic Review and Meta-Analysis.

Introductions: The diet during pregnancy has long-term effects on the health outcomes of both mothers and their children. Study aimed to undertake a systematic review to explore the association of high-fiber diet, DASH diet, and Mediterranean diet with metabolic outcomes among mothers and their children.

Methods: We searched for relevant articles published from Jan 2012 to Nov 2022 through PubMed, MEDLINE and Embase.

Diagnostic performance of multiparametric nonenhanced magnetic resonance imaging (MRI) in grading glioma and correlating IDH mutation status.

Aim: To evaluate the diagnostic performance of nonenhanced magnetic resonance imaging (MRI) in grading glioma and correlating isocitrate dehydrogenase (IDH) mutation status.

Materials And Methods: Patients with diagnoses confirmed by postoperative pathology were enrolled. Quantitative parameters, including the relative amide proton transfer-weighted (rAPTW), relative cerebral blood flow (CBF), and apparent diffusion coefficient (ADC) were applied to grade gliomas and correlate IDH mutation status.

Odor impact patterns and health risks of various enterprises in the rubber product manufacturing industry.

The rubber product manufacturing (RPM) industry generates a large number of odor complaints because persistent and distinctive volatile compounds are released during the associated processes. Such compounds represent a nuisance and may pose health risks to nearby residents. Extensive monitoring and sampling identified 146 volatile organic compounds (VOCs) from 20 enterprises across 6 subcategories of the RPM industry.