Effect of tolfenamic acid on the metabolism of the main connective tissue components in rats.

Tolfenamic acid (Clotam) has been used in the therapy of rheumatic diseases for some years. Regarding its chemical structure it belongs to the group of fenamates. The effect of tolfenamic acid on the synthesis of collagen and proteoglycans in granulation tissue, skin or cartilage of rat weanlings was tested and compared with the action of mefenamic acid.

Protein binding of tolfenamic acid in the plasma from patients with renal and hepatic disease.

The protein binding of tolfenamic acid in plasma from patients with renal and hepatic disorders was studied by equilibrium dialysis. Drug binding to the cellular components of whole blood and blood cell suspensions was also measured. Salicylic acid was used as the reference drug in all experiments.

Comparison of saliva and urine samples in thin-layer chromatographic detection of central nervous stimulants.

The excretion of amphepramone, amphetamine, ephedrine and prolintane was estimated qualitatively from saliva and urine using thin-layer chromatography (TLC) 1, 2, 4, 8, 12 and 24 h after a single intake of therapeutic doses by healthy volunteers. The purpose was to compare the suitability of these two biological fluids for doping tests and it was therefore important that either the drug or its metabolites should be found in all subjects. Ephedrine was the only one to be detected regularly in saliva already 1 h after drug intake, and it was found there up to 8 h, while in urine it was found up to 24 h.

Intestinal absorption of tolfenamic acid at experimental malabsorption states in rats.

Absorption kinetics of 14C-labelled N-(3-chloro-o-tolyl)-anthranilic acid (tolfenamic acid, 14C-TA, Clotam) from the small intestine was studied in intact rats and in rats with malabsorption states provoked by methotrexate, starvation, and triparanol. 14C-TA was administered intravenously and intraduodenally, and the drug concentrations in the blood were followed up radiometrically. A multi-compartmental model was applied for mathematical analysis.

ATPase stimulated by Na+ or K+ in gills of the freshwater mussel Anodonta.

1. Microsomal preparations from the gills of the freshwater mussel anodonta cygnea cellensis show Mg2+ -dependent Na+ - or K+ -stimulated ATPase activity, which is not inhibited by ouabain. 2.

Weight, stroma to gland ratio, and mitotic activity of the human hyperplastic prostate.

The growth of the hyperplastic prostate of 12 patients was studied with an in vitro 3H-thymidine labeling method. The prostates were weighed and the stroma to gland ratio was analyzed. The mitotic activity of the glandular epithelium averaged 0.

A simple method for the clinical determination of the mitotic activity of the human prostate in vitro.

Patients with a hyperplastic prostate were studied for the epithelial mitotic activity of adenomatous tissue by the (3)H-thymidine labelling method. A simple method in vitro was improved for biopsy samples. The mitotic activities obtained in vitro were compared with those obtained in vivo.