A root nodule microbiome sequencing data set from red alder (Alnus rubra Bong.).

There have been frequent reports of more than one strain of the nitrogen-fixing symbiont, Frankia, in the same root nodule of plants in the genus Alnus, but quantitative assessments of their relative contributions have not been made to date. Neither has the diversity of other microbes, having potential functional roles in symbiosis, been systematically evaluated. Alnus rubra root nodule microbiota were studied using Illumina short read sequencing and kmer-based read classification.

Four new species and new records of Macrostomus Wiedemann (Diptera: Empididae) from Northern and Northeastern Regions of Brazil.

Macrostomus Wiedemann is recorded for the first time in the Cerrado Biome and also from the state of Maranhão, Brazil. The following species are treated here: Macrostomus maranhensis sp. nov.

Antioxidant Activity, Antiproliferative Activity, Antiviral Activity, NO Production Inhibition, and Chemical Composition of Essential Oils and Crude Extracts of Leaves, Flower Buds, and Stems of .

The chemical composition of extracts (CEs) and essential oils (EOs) from leaves, flower buds, and stems was analyzed. Antiproliferative activity against tumor cell lines, NO production inhibition, and antioxidant and antiviral activities were assessed. The CEs contained flavonoids, phenolic acids, coumarins, and saturated fatty acids.

Learning needs and the orientation received by patients in the preoperative period of myocardial revascularization.

Objective: To identify the learning needs and the orientations received by patients in the preoperative period of coronary artery bypass surgery (CABG).

Method: Descriptive study, with a qualitative approach. Data collection took place from January to April 2021, through interviews carried out with 13 pre-operative patients admitted to the cardiovascular unit of a university hospital in Northeastern Brazil.

Wearable sensors in patient acuity assessment in critical care.

Acuity assessments are vital for timely interventions and fair resource allocation in critical care settings. Conventional acuity scoring systems heavily depend on subjective patient assessments, leaving room for implicit bias and errors. These assessments are often manual, time-consuming, intermittent, and challenging to interpret accurately, especially for healthcare providers.

Comparative analysis of basal and etoposide-induced alterations in gene expression by DNA-PKcs kinase activity.

Maintenance of the genome is essential for cell survival, and impairment of the DNA damage response is associated with multiple pathologies including cancer and neurological abnormalities. DNA-PKcs is a DNA repair protein and a core component of the classical nonhomologous end-joining pathway, but it also has roles in modulating gene expression and thus, the overall cellular response to DNA damage. Using cells producing either wild-type (WT) or kinase-inactive (KR) DNA-PKcs, we assessed global alterations in gene expression in the absence or presence of DNA damage.

Diurnal Pain Classification in Critically Ill Patients using Machine Learning on Accelerometry and Analgesic Data.

Quantifying pain in patients admitted to intensive care units (ICUs) is challenging due to the increased prevalence of communication barriers in this patient population. Previous research has posited a positive correlation between pain and physical activity in critically ill patients. In this study, we advance this hypothesis by building machine learning classifiers to examine the ability of accelerometer data collected from daily wearables to predict self-reported pain levels experienced by patients in the ICU.

Identification and quantitation of multiple variants in RNA virus genomes.

The goal of the study was to identify and characterize RNA virus variants containing mutations spread over genomic distances >5 kb. As proof of concept, high-quality viral RNA of the Dengue 2 component of Takeda's tetravalent dengue vaccine candidate (TDV-2) was used to develop a reverse transcription-polymerase chain reaction protocol to amplify a ∼5.3 kb cDNA segment that contains the three genetic determinants of TDV-2 attenuation.

Complexes of species of Eumorpha Hübner [1807] (Lepidoptera: Sphingidae) from the Maranhão State, Brazil.

Eumorpha is a genus of moths that was described by Hübner in 1807, and currently has 28 valid species, distributed between temperate and tropical regions of the Americas. Thirteen species have been recorded for Brazil. The main characteristic of the group is the presence of a dark rectangular bar on the forewing of most species and also a pair of triangular spots on the thorax.

Annotated genome sequence of a fast-growing diploid clone of red alder (Alnus rubra Bong.).

Red alder (Alnus rubra Bong.) is an ecologically significant and important fast-growing commercial tree species native to western coastal and riparian regions of North America, having highly desirable wood, pigment, and medicinal properties. We have sequenced the genome of a rapidly growing clone.

A review on hydrothermal carbonization of potential biomass wastes, characterization and environmental applications of hydrochar, and biorefinery perspectives of the process.

It is imperative to search for appropriate processes to convert wastes into energy, chemicals, and materials to establish a circular bio-economy toward sustainable development. Concerning waste biomass valorization, hydrothermal carbonization (HTC) is a promising route given its advantages over other thermochemical processes. From that perspective, this article reviewed the HTC of potential biomass wastes, the characterization and environmental utilization of hydrochar, and the biorefinery potential of this process.

Gender Parity in Geriatrics Editorial Boards.

Gender equality, with an emphasis on female education, has been designated by the United Nations as one of the 17 Sustainable Development Goals (SDGs) to be completed by 2030, since gender disparity is a major impediment to scientific and economic progress. This study was carried out in an effort to address the gender gaps that can be seen in academic and scientific publications. The purpose of this study is to describe the gender distribution of editorial board members and editors-in-chief across geriatrics journals with high impact factors.

Ethnomedicinal, Phytochemical and Pharmacological Investigations of (Hochst.) Codd (Lamiaceae).

Hochsteter codd. () in its native African continent, is considered one of the most popular aromatic medicinal plants. In folk medicine it may be used as an infusion to treat respiratory problems, cough, headache, stomach pain, diarrhea, fever, malaria, and dengue; and in the form of compresses it is applied for the relief of headaches and toothaches.

Epidemiological characterization of notified human brucellosis cases in Southern Brazil.

Brucellosis is one of the most important and widespread bacterial zoonotic diseases worldwide, and it is transmitted to humans from various sources, including direct contact with infected animals and the ingestion of contaminated products, including unpasteurized milk. There are only a few epidemiological studies on said disease in humans in Western Santa Catarina, a region instantiated by agriculture. Thus, the objective of this study was to characterize the epidemiological aspects of human brucellosis reported in Western Santa Catarina from 2013 to 2018.

Factors That Impact the Decision to Perform Left Ventriculography in Coronary Artery Disease.

Background: Left ventriculography is an invasive method for assessment of left ventricular systolic function. Since the advent of noninvasive methods, its use has been questioned, as it carries some risk to the patient.

Objective: To assess which factors are independently associated with the decision to perform ventriculography in patients with coronary artery disease.

Contiguous Genome Sequence of sp. Strain ArI3, Isolated from Root Nodules of Alnus rubra Bong.

We report the genome sequence of sp. strain ArI3, recovered as a single contig from one run of the Oxford Nanopore Technologies (ONT) MinION instrument. The genome has a G+C content of 72%, is 7,541,222 bp long, and contains 5,427 predicted protein-coding genes.

Short-term high fat diet alters genes associated with metabolic and vascular dysfunction during adolescence in rats: a pilot study.

Background: Diet-induced metabolic dysfunction precedes multiple disease states including diabetes, heart disease, and vascular dysfunction. The critical role of the vasculature in disease progression is established, yet the details of how gene expression changes in early cardiovascular disease remain an enigma. The objective of the current pilot project was to evaluate whether a quantitative assessment of gene expression within the aorta of six-week old healthy male Sprague-Dawley rats compared to those exhibiting symptoms of metabolic dysfunction could reveal potential mediators of vascular dysfunction.

Bio-activating ultrafine grain titanium: RNA sequencing reveals enhanced mechano-activation of osteoconduction on nanostructured substrates.

Titanium is essentially absent from biological systems yet reliably integrates into bone. To achieve osseointegration, titanium must activate biological processes without entering cells, defining it as a bio-activating material. Nanostructuring bulk titanium reduces grain size, increases strength, and improves other quantifiable physical properties, including cytocompatibility.

Identification of regenerative processes in neonatal spinal cord injury in the opossum (Monodelphis domestica): A transcriptomic study.

This study investigates the response to spinal cord injury in the gray short-tailed opossum (Monodelphis domestica). In opossums spinal injury early in development results in spontaneous axon growth through the injury, but this regenerative potential diminishes with maturity until it is lost entirely. The mechanisms underlying this regeneration remain unknown.

Effect of surface grain boundary density on preosteoblast proliferation on titanium.

Studies since 2004 have shown that the cytocompatibility of ultrafine grain (UG) commercial purity (CP) titanium exceeds that of coarse grain (CG) CP titanium (Ti) by 30% to 20-fold. To isolate the factors affecting this large reported variability of CP titanium's cytocompatibility, discs of UG and CG titanium were fabricated with controlled texture and roughness. The discs were seeded with MC3T3-E1 pre-osteoblastic cells and cultured for 72 h.

Validation of educational material for the care of people with intestinal stoma.

Objective: to validate an educational booklet for people with intestinal stoma as a technological resource in the teaching of self-care.

Method: a methodological research for the construction and validation of an educational booklet by nine expert judges and 25 people with stomas. The agreement index of at least 80% was considered to guarantee the validation of the material.

Cutaneous leishmaniasis mimicking cutaneous lymphoma.

Cutaneous leishmaniasis (CL) is caused by protozoa of genus that are transmitted to humans through the bite of sand flies ( and ). The infection is classically manifested as multiple or single ulcers affecting cutaneous and/or mucosal areas of the body. Atypical lesions are relatively uncommon, being able to simulate a large variety of benign and malign dermatological disorders.