Damage analysis and optimal design of micro-structure milling cutter based on peridynamics.

H13 die steel has the characteristics of high hardness, strong toughness, and good heat resistance, and is a typical difficult to process materials material. During the cutting process, it is prone to accelerate tool wear and cause thermal deformation. By reasonably designing micro-grooves, the comprehensive performance of the tool can be effectively improved.

Recombinant oncolytic adenovirus armed with CCL5, IL-12, and IFN-γ promotes CAR-T infiltration and proliferation in vivo to eradicate local and distal tumors.

The efficacy of chimeric antigen receptor T (CAR-T) cells for solid tumors remains unsatisfactory due to the limited tumor infiltration and immunosuppressive microenvironment. To overcome these limitations, the genetically engineered recombinant oncolytic adenoviruses (OAVs) that conditionally replicate in tumor cells were developed to modify the tumor microenvironment (TME) to facilitate CAR-T-mediated tumor eradication. Here in the present study, a novel recombinant OAV carrying CCL5, IL12, and IFN-γ controlled by Ki67 promoter was constructed (named AdKi67-C3).

Research on constrained localization of ultrasound geometric distribution based on FHN neurons.

The autopilot positioning process is mainly affected by three aspects: the first is the spatial geometric distribution of positioning sensors; the second is the screening of spurious observations; and the third is the equivalent ranging error. A constrained positioning method based on the geometric distribution of FitzHugh-Nagumo (FHN) neurons is proposed. To reduce the geometric accuracy factor, a Horizontal Dilution Of Precision value algorithm with a weight factor was proposed by considering the spatial geometric distribution of base stations and the geometric relationship of anchor points.

HBV X Protein Induces Degradation of UBXN7, a Novel Negative Regulator of NF-κB Signaling, to Promote HBV Replication.

Chronic hepatitis B virus (HBV) infection is a leading cause of hepatocellular carcinoma. However, the function and mechanism of the effect of HBV on host protein ubiquitination remain largely unknown. We aimed at characterizing whether and how HBV promotes self-replication by affecting host protein ubiquitination.

Upregulation of miR-520c-3p via hepatitis B virus drives hepatocellular migration and invasion by the PTEN/AKT/NF-κB axis.

Hepatitis B virus (HBV) is a major risk factor for the development and progression of hepatocellular carcinoma (HCC). It has been reported that viral infection can interfere with the expression of cellular microRNA (miRNA) to affect oncogenesis. In this study, we showed that miR-520c-3p was upregulated in liver tumor specimens, and we revealed that HBV infection enhanced the expression of miR-520c-3p through the interaction of viral protein HBV X protein (HBx) with transcription factor CREB1.

Triple-serotype chimeric oncolytic adenovirus exerts multiple synergistic mechanisms against solid tumors.

Background: Oncolytic virotherapy has become an important branch of cancer immunotherapy. This study investigated the efficacy of an oncolytic adenovirus (OAV), OncoViron, with synergistic mechanisms in the treatment of multiple solid tumors.

Methods: An OAV, OncoViron, was constructed and investigated by cytological experiments and implanted tumor models of multiple solid tumor cell lines to certify its anticancer efficacy, the synergistic effects of viral oncolysis and transgene anticancer activity of OncoViron, as well as oncolytic virotherapy combined with immunotherapy, were also verified.

Multiomics Analysis of Endocytosis upon HBV Infection and Identification of SCAMP1 as a Novel Host Restriction Factor against HBV Replication.

Hepatitis B virus (HBV) infection remains a major global health problem and the primary cause of cirrhosis and hepatocellular carcinoma (HCC). HBV intrusion into host cells is prompted by virus-receptor interactions in clathrin-mediated endocytosis. Here, we report a comprehensive view of the cellular endocytosis-associated transcriptome, proteome and ubiquitylome upon HBV infection.

Oncolytic adenovirus-mediated expression of decorin facilitates CAIX-targeting CAR-T therapy against renal cell carcinoma.

Although chimeric antigen receptor T cell (CAR-T) therapy has been successful for hematological malignancies, it is less effective for solid tumors. The primary reason is that the immune microenvironment restricts CAR-T cells from infiltrating and proliferating in tumors. Oncolytic virotherapy has emerged as a novel immunogenic therapy to augment antitumor immune response.

Influence of Microgroove Structure on Cutting Performance and Chip Morphology during the Turning of Superalloy Inconel 718.

This study designed a new microgroove cutting tool to machine Inconel 718 and focused on the effect of microgroove structure on the cutting performance and chip morphology during the turning. A comparative analysis of the cutting force, cutting temperature, tool life, tool wear, and chip morphology of the microgroove cutting tool and the original cutting tool was conducted. The main cutting force and temperature of the microgroove cutting tool were reduced by 12% and 12.

Forming process and evaluation of chip in machining of high-strength steel by an independent-developed microgroove turning tool.

Chip morphology is one of the evaluation indexes of cutting performance of cutting tools, and chip forming process has a direct and important influence on chip morphology. High-strength steel 40CrMnMo is one of typical oil country tubular goods (OCTG) and difficult-to-cut materials, and its chip morphology represents the machining quality of OCTG. The chip forming process of a new independent-developed microgroove turning tool for turning oil country tubular goods 40CrMnMo is researched, combining machining experiments with theoretical analysis.

Global analysis of HBV-mediated host proteome and ubiquitylome change in HepG2.2.15 human hepatoblastoma cell line.

Hepatitis B virus (HBV) infection remains a major health issue worldwide and the leading cause of cirrhosis and hepatocellular carcinoma (HCC). It has been reported previously that HBV invasion can extensively alter transcriptome, the proteome of exosomes and host cell lipid rafts. The impact of HBV on host proteins through regulating their global post-translational modifications (PTMs), however, is not well studied.

Suppression of HIV-1 Integration by Targeting HIV-1 Integrase for Degradation with A Chimeric Ubiquitin Ligase.

Human immunodeficiency virus (HIV) attacks human immune system and causes life-threatening acquired immune deficiency syndrome (AIDS). Treatment with combination antiretroviral therapy (cART) could inhibit virus growth and slow progression of the disease, however, at the same time posing various adverse effects. Host ubiquitin-proteasome pathway (UPP) plays important roles in host immunity against pathogens including viruses by inducing degradation of viral proteins.

ABIN1 inhibits HDAC1 ubiquitination and protects it from both proteasome- and lysozyme-dependent degradation.

ABIN1, an important immune regulator, has been shown to be involved in various cellular functions, such as immunity, development, tissue homeostasis, and tumor progression. It inhibits TNF- and TLR-induced NF-κB signaling activation and the consequent gene expression. Despite its functional significance, the mechanism of ABIN1 in the regulation of various cellular functions remains unclear.

Multilinear Spatial Discriminant Analysis for Dimensionality Reduction.

In the last few years, great efforts have been made to extend the linear projection technique (LPT) for multidimensional data (i.e., tensor), generally referred to as the multilinear projection technique (MPT).

New taxa of (Berberidaceae) with greenish flowers from a biodiversity hotspot in Sichuan Province, China.

A new species, X. H. Li, and a new variety, Franch.