Dysregulation of stress erythropoiesis and enhanced susceptibility to Salmonella Typhimurium infection in AhR-deficient mice.

Background: By acting as an environmental sensor, the ligand-induced transcription factor aryl hydrocarbon receptor (AhR) regulates acute innate and adaptive immune responses against pathogens. Here, we analyzed the function of AhR in a model for chronic systemic infection with attenuated Salmonella Typhimurium (STM).

Methods: WT and AhR-deficient mice were infected with the attenuated STM strain TAS2010 and analyzed for bacterial burden, host defense functions and inflammatory stress erythropoiesis.

CCL17-expressing dendritic cells in the intestine are preferentially infected by Salmonella but CCL17 plays a redundant role in systemic dissemination.

Introduction: Salmonella spp. are a recognized and global cause of serious health issues from gastroenteritis to invasive disease. The mouse model of human typhoid fever, which uses Salmonella enterica serovar Typhimurium (STM) in susceptible mouse strains, has revealed that the bacteria gain access to extraintestinal tissues from the gastrointestinal tract to cause severe systemic disease.

A randomized, open-label, multicenter, comparative study of therapeutic efficacy, safety and tolerability of BNO 1030 extract, containing marshmallow root, chamomile flowers, horsetail herb, walnut leaves, yarrow herb, oak bark, dandelion herb in the treatment of acute non-bacterial tonsillitis in children aged 6 to 18 years.

Unlabelled: Seventy to 95% of acute tonsillitis episodes are caused by viral infection, therefore why antibiotic therapy is not indicated in majority of cases. In such cases, acetaminophen or ibuprofen are used to alleviate the symptoms. The objective of this study was assessment of efficacy of phytoneering extract BNO 1030 (Imupret®) in patients with acute non-bacterial tonsillitis.

Accumulation of novel glycolipids and ornithine lipids in Mesorhizobium loti under phosphate deprivation.

Glycolipids are found mainly in photosynthetic organisms (plants, algae, and cyanobacteria), Gram-positive bacteria, and a few other bacterial phyla. They serve as membrane lipids and play a role under phosphate deprivation as surrogates for phospholipids. Mesorhizobium loti accumulates different di- and triglycosyl diacylglycerols, synthesized by the processive glycosyltransferase Pgt-Ml, and two so far unknown glycolipids, which were identified in this study by mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy as O-methyl-digalactosyl diacylglycerol (Me-DGD) and glucuronosyl diacylglycerol (GlcAD).

A bifunctional glycosyltransferase from Agrobacterium tumefaciens synthesizes monoglucosyl and glucuronosyl diacylglycerol under phosphate deprivation.

Glycolipids are mainly found in phototrophic organisms (like plants and cyanobacteria), in Gram-positive bacteria, and a few other bacterial phyla. Besides the function as bulk membrane lipids, they often play a role under phosphate deprivation as surrogates for phospholipids. The Gram-negative Agrobacterium tumefaciens accumulates four different glycolipids under phosphate deficiency, including digalactosyl diacylglycerol and glucosylgalactosyl diacylglycerol synthesized by a processive glycosyltransferase.

[Treatment of urinary incontinence in postmenopausal women].

Examination of 65 postmenopausal women with urinary incontinence demonstrated that most of the patients had mixed urinary incontinence. Pathogenesis of urinary incontinence in postmenopausal women is strongly associated with estrogen deficiency. This dictates necessity of preoperative hormone replacement therapy.

Carotenoids and carotenoproteins in Asellus aquaticus L. (Crustacea: Isopoda).

Column (CC), thin-layer (TLC), high-performance liquid (HPLC) and ion-exchange chromatography (IEC), were used to investigate corotenoid and carotenoprotein complexes in Asellus aquaticus specimens from the Narew river. The following carotenoids were found: alpha-carotene, beta-carotene, beta-cryptoxanthin, lutein, zeaxanthin, diadinoxanthin, mutatoxanthin, crustaxanthin, echinenone, hydroxyechinenone, phoenicoxanthin, canthaxanthin and astaxanthin. Astaxanthin (37.

[Isosteric triphosphonate analogues of dNTP: synthesis and substrate properties toward various DNA polymerases].

Isosteric triphosphonate derivatives of 2',3'-dideoxy-2',3'-didehydroadenosine and 3'-deoxy-2',3'-didehydrothymidine and their beta,gamma-substituted analogues were synthesized. Their substrate properties toward a number of reverse transcriptases of the human immunodeficiency and bird myeloblastosis viruses, human DNA polymerases alpha and beta, and the Klenow fragment of Escherichia coli DNA polymerase I were studied.

[Treatment of patients with urogenital prolapse and urine incontinence].

Treatment outcomes are reviewed for 58 females aged 36 to 54 years with genital prolapse (GP) and urine incontinence (UI): colpoptosis (n=44), incomplete falling of the womb (n=14), UI type IIa (n=36), UI type IIb (n=22). All the patients received surgical treatment. Preoperative biological biofeedback-therapy was used.

[Diagnosis and treatment of upper urinary tract infection in women as a postoperative complication for treating genital endometriosis].

156 women operated on for genital endometriosis received treatment for infection of the upper urinary tracts diagnosed by ultrasound. The treatment was conducted only in case of normal urodynamics achieved by drainage (on demand) and comprised antibacterial therapy, efferent hemocorrection, intravascular laser irradiation of blood, hyperbaric oxigenation. A response was obtained in 98% patients.

[The effect of the activation of lipid peroxidation on the structure of extraembryonic organs and the liver in rats at different periods of ontogeny].

The influence of activation of lipid peroxidation (LP) on morphofunctional organization of extraembryonic compounds and liver on different stages of rat's ontogenesis was studied. It is found that LP-activation (paraquat injection) in late pregnancy leads to extensive vacuolization of trophoblastic and yolk sac cells. In the embryonic liver, the number of hemopoietic cells decreases.

[The antagonistic activity of the bacterial flora in feed in relation to the causative agent of diarrhea in calves].

Antagonistic activity of 264 strains (6 species) of aerobic bacilli as well as 45 strains (3 species) of bacteria from genera Chromobacterium and Pseudomonas has been studied. They were isolated from the fodder at the farms of Ukraine five regions in 1986-1992 in respect to 128 strains (17 species) of genera Escherichia, Proteus, Salmonella, Shigella and Pasteurella, isolated by the authors from feces of calf with symptoms of diarrhea. High antagonistic activity of Bacillus polymyxa 168 (zone of growth inhibition of test cultures of 10-20 mm) in respect of diarrhea agents was established.

[Changes in the hepatocytes of adult male rats subjected to the action of 3,4-benzopyrene at different periods of ontogeny].

The effects of benzpyrene (BP) were studied in 32 male Wistar rats. Treatment of intact mature animals with benzpyrene causes transformations of hepatocytes. Antenatal treatment with BP initiates a complex of compensatory-adaptational reactions in the liver of progeny and the effect retains at the postnatal period.

[Neuromediator provision of the organs of immune system in benzpyrene intoxication].

The density of adrenergic innervation and relative content of catecholamines were investigated in rat lymphoid organs under different type of benzpyrene treatment. It was found that both ante- and postnatal influence of toxicant leads to a decrease of neurotransmitter providing of the thymus, spleen, mesenterial, iliac and popliteal lymph nodes. On the contrary, when mixed ante- and postnatal benzpyrene influence has place, the adrenergic innervation density and relative content of catecholamines are increased.

[Antioxidants in the combined treatment of acute cholecystitis in middle-aged and elderly patients].

Investigations of the state of processes of lipid peroxidation (PLP) and the antioxidant system (AOS) of the organism performed in 267 elderly and senile patients have shown destabilization of the system "PLP-AOS". The correction of processes of lipid peroxidation with antioxidants improved the course of the postoperative period, decreased the amount of complications and lethality. It allowed a conclusion of the necessity to use antioxidants in the complex treatment of such patients.

[Morphological evidence of the possible role of cytochrome P-450 in the development of an autoimmune process in the liver].

Morphological alterations in rat's liver during lipid peroxidation induction by paraqat were studied. Infiltration of liver by lymphocytes, macrophages and plasma cells was studied. On ultrastructure level profound extension of rough reticulum cistern and destruction of mitochondrial cristae were revealed by immunohistochemical methods.

[Treatment of patients with genital endometriosis and nephroptosis].

The authors analyze the results of multiple-modality treatment of 31 patients suffering from genital endometriosis combined with nephroptosis. Nephropexy with a muscular flap was carried out in all the cases, followed by therapy for genital endometriosis, including hormones, immunomodulators, performed physical factors, hyperbaric oxygenation, etc. Late results were followed up for up to 3 years.

[The characteristics of glutathione reductase distribution and activity in the liver of adult rats subjected to paraquat action in the embryonic period].

Paraquat action on glutathione reductase activity and intratissue distribution in the liver of intact rats and also in the animals antenatally treated with the given herbicide was studied by some biochemical and histochemical methods. It has been ascertained that paraquat injection into intact animals promotes the increase in the intracellular enzyme level, development of glutathione reductase staining of hepatocyte nuclei, diffuse distribution of the staining along the lobe. In rats antenatally treated with paraquat acute priming does not induce glutathione reductase.

[X-ray diagnosis of urinary tract diseases in genital endometriosis].

The paper is concerned with the results of combined investigation of 157 women suffering from genital endometriosis, operated on for this disease. The involvement of the lower parts of the ureters in a pathological process was detected in 48 patients before operation and in 6 patients at operation. Comparison of x-ray and operative findings with histology specimens made it possible to define endogenous and exogenous forms of ureteral endometriosis and three variants of an x-ray picture in this pathology (the absence of abnormal changes of the urinary tract, hydroureter is over the narrowed part of the ureter, hydroureteronephrosis).

[Radiologic diagnosis of urinary tract disorders in genital endometriosis].

The paper is concerned with the results of combined investigation of 157 women suffering from genital endometriosis, operated on for this disease. The involvement of the lower parts of the ureters in a pathological process was detected in 48 patients before operation and in 6 patients at operation. Comparison of x-ray and operative findings with histology specimens made it possible to define endogenous and exogenous forms of ureteral endometriosis and three variants of an x-ray picture in this pathology (the absence of abnormal changes of the urinary tract, hydroureter is over the narrowed part of the ureter, hydroureteronephrosis).

[The involvement of the urinary system organs in women suffering from genital endometriosis].

The data on the complicated character of endometriosis treatment, in females with the involved urinary system in particular, were discussed. The reported incidence of the affected urinary system in females with endometriosis ranged from 1-2 to 18.5 per cent.

[Effect of low-molecular toxic substances on the organs of the immune system in mothers and their offspring].

Administration of benzpirene to the mother is accompanied with activation of B-and decrease in T-links of immune activity both in the mother and offspring. The lymphoid organs in the animals, not subjected antenatally to benziprene action, but given the substance after birth, undergo destabilization and are not ready to administration of this polycyclic hydrocarbonic compound. This produces their hypoplasia.

[Metabolism of antipyrine during pregnancy].

Dynamics of the saliva antipyrine content in women throughout the period of pregnancy was studied. There were estimated the half-life of antipyrine in the saliva, elimination constant, total clearance, apparent volume of distribution, area below pharmacokinetic curve.