Publications by authors named "Selma N Takajjart"
Article Synopsis
- Cellular senescence is linked to aging and diseases, but measuring senescent cells is difficult due to a lack of specific markers and methods.
- The Fully-Automated Senescence Test (FAST) is introduced as an efficient, image-based technique to evaluate senescence in cultured cells by assessing key markers like SA-β-Gal activity, proliferation arrest, and cell morphology.
- FAST offers standardized, automated imaging and data analysis, minimizing false positives, and enables large-scale experiments in aging research by accurately quantifying senescence across various cell types.
Cellular senescence is a major driver of aging and age-related diseases. Quantification of senescent cells remains challenging due to the lack of senescence-specific markers and generalist, unbiased methodology. Here, we describe the Fully-Automated Senescence Test (FAST), an image-based method for the high-throughput, single-cell assessment of senescence in cultured cells.
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