Postexposure subunit vaccination against chronic enteric mycobacterial infection in a natural host.

The control of chronic bacterial diseases with high prevalence in areas of endemicity would strongly benefit from availability of postexposure vaccines. The development of these vaccines against mycobacterial infections, such as (para)tuberculosis, is hampered by lack of experience in natural hosts. Paratuberculosis in cattle is both a mycobacterial disease of worldwide importance and a natural host model for mycobacterial infections in general.

Evaluation of a LPS-based glycoconjugate vaccine against bovine Escherichia coli mastitis: Formation of LPS Abs in cows after immunization with E. coli core oligosaccharides conjugated to hemocyanine.

The immune response of cows against the core oligosaccharide of Escherichia coli rough mutants (core types R1-R4, K-12 and J-5) was investigated after immunization with a synthetic glycoconjugate composed of deacylated LPS conjugated to hemocyanine (22 animals). Ab formation was measured by ELISA using LPS or deacylated LPS conjugated to BSA as an Ag. The glycoconjugate immunogens were used to vaccinate cows (36 animals), which were then challenged intramammarily with E.

Identification of specific genes in Staphylococcus aureus strains associated with bovine mastitis.

Staphylococcus aureus is a common cause of bovine mastitis that is responsible for the main economic loss to the dairy industry. For identification of putative, bovine-specific molecular marker a genome comparison between bovine S. aureus strain RF122 and 52 previously sequenced S.

Heat shock protein 70 subunit vaccination against bovine paratuberculosis does not interfere with current immunodiagnostic assays for bovine tuberculosis.

Paratuberculosis or Johne's disease in ruminants is an infectious disease of the small intestine caused by Mycobacterium avium spp. paratuberculosis, and a global problem of the livestock industry. No therapy is available and the use of a whole bacterin vaccine is limited due to interference with tuberculosis diagnostics.