Objectives: To monitor the spread of invasive meningococcal disease due to group C of the clonal complex 10217 isolates beyond the sub-Saharan African meningitis belt.
Methods: Cases were confirmed by real-time polymerase chain reaction in blood or cerebrospinal fluid samples and further characterized by multi-locus sequence typing that defined sequence type and clonal complexes. Sequencing of gene (encoding the penicillin-binding protein 2) was also used to predict susceptibility to β-lactams.
Objectives: We implemented a project named MENINGSTOP in three countries of North Africa (Algeria, Morocco and Tunisia). The main objective was to use real-time PCR to detect, identify and type the three main agents (Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae) responsible for invasive bacterial infections (IBI).
Methods: The protocol of WHO and US CDC for real-time PCR was used to detect and type the three bacterial agents in clinical samples.