Color Correction of Stained Tissue Section Images by Histogram Transfer According to Control Images.

Objective: To present a color correction method by histogram transfer depending upon control tissue image (CTI) differences, and subsequently evaluate its performance.

Study Design: Images from colon and placenta sections stained by anti-CD34 were used as CTI and/ or sample tissue images (STIs). In total, 36 slides were stained: 1 according to standard procedure and 35 with some variation in the durations or dilutions used for the staining process.

A review on color normalization and color deconvolution methods in histopathology.

The histopathologists get the benefits of wide range of colored dyes to have much useful information about the lesions and the tissue compositions. Despite its advantages, the staining process comes up with quite complex variations in staining concentrations and correlations, tissue fixation types, and fixation time periods. Together with the improvements in computing power and with the development of novel image analysis methods, these imperfections have led to the emerging of several color normalization algorithms.