Sutureless microvascular anastomosis with the aid of heparin loaded poloxamer 407.

Background: Anastomosis with tissue adhesives is an alternative method for conventional anastomosis. However, this method has several technical challenges. It requires the use of suture to prevent leakage into lumen and precise application onto all surfaces of the anastomosis site.

The Effects of Hydrogen Sulfide on Adipocyte Viability in Human Adipocyte and Adipocyte-Derived Mesenchymal Stem Cell Cultures Under Ischemic Conditions.

Background: This study evaluated the in vitro effects of hydrogen sulfide on adipocyte survival under ischemic conditions and explored possible mechanisms of its apoptotic process.

Methods: The mesenchymal stem cell culture was prepared from a human subcutaneous adipose tissue sample. Adipose-derived mesenchymal stem cells were differentiated into the adipogenic direction, and a mature adipocyte culture was obtained.

Reflectance confocal microscopy as a useful diagnostic tool for monitoring of skin containing vascularized composite allograft rejection: A preliminary study on rats.

Vascularized composite allografts can undergo immune-mediated rejection, and skin biopsies are needed for monitoring of the transplant. However it is an invasive method, and requires processing time and pathological assessment. The purpose of this study is to use a new noninvasive monitoring method of the reflectance confocal microscopy (RCM) to determine severity of the allograft rejection on rats.

Palmar contracture release with arterialized venous instep flap: An anatomical and clinical study.

Background: Plantar skin has similar histologic features to the palmar area and appears to be the ideal tissue for reconstruction of the palmar region. In this study, an anatomic examination was performed to determine the superficial venous architecture of the instep area, and the use of arterialized venous instep flaps for palmar contracture release was assessed.

Methods: The anatomical study was performed on 12 fresh cadaver feet.

Evaluation of the results of simultaneous open rhinoplasty and Abbe flap for the reconstruction of the secondary bilateral cleft and nasal deformity.

Aims: We aimed to evaluate the results of simultaneous rhinoplasty and Abbe flap for the reconstruction of the secondary bilateral cleft and nasal deformity by means of a newly developed software-based method.

Patients And Methods: A total of 16 patients with the bilateral cleft lip nasal deformity received Abbe flap and simultaneous open rhinoplasty between 2004 and 2010. The mean age of the patients was 21 years, and the average follow-up time was 2.

Reconstruction of the one-digit foot with an osteocutaneous fibula flap: a case report.

The decision to amputate or reconstruct after high-energy foot injuries is controversial. A 25-year-old male patient was admitted to our clinic with a complex injury to his left foot sustained during a mine explosion, and the second to fifth digits and metatarsals of the left foot had been traumatically amputated before admission to our facility. The complex left foot defect was reconstructed with an osteocutaneous fibula flap during a single session.

A new approach to objective evaluation of the success of nasal septum perforation.

Background: Perforations in the nasal septum (NSP) give rise not only to disintegration of the septum anatomy but also impairment in normal nasal physiology. The successes of these surgical techniques are usually equated to anatomical closure of the perforation. The goal of this study is to evaluate the subjective and objective results of our surgical technique for septal perforation surgery.

Auto-/homografting can work well even if both autograft and allograft are meshed in 4:1 ratio.

Background: Patients suffering major burns of more than 50% total burn surface area lack an adequate skin graft donor site to resurface extensive burn wounds and usually need widely meshed autografting and allografting. Anything over the 3:1 expansion ratio is strongly associated with low graft take, poor or delayed epithelialization, and hypertrophic scarring.

Methods: In this study, both autografts and allografts were expanded at a 4:1 ratio.

Experimental assessment of the neo-vascularisation of acellular dermal matrix in the wound bed pretreated with mesenchymal stem cell under subatmospheric pressure.

Neo-vascularisation of the acellular dermal matrix (ADM) is an essential procedure if a full-thickness wound is closed with ADM and skin is grafted over the ADM. In this study, we aimed to improve the neo-vascularisation of ADM by combining the effects of negative pressure wound therapy (NPWT) and mesenchymal stem cells (MSCs) on angiogenesis. In this study, 28 female Sprague-Dawley rats were used and divided into four groups.

Biomechanical analysis of the effect of mesenchymal stem cells on mandibular distraction osteogenesis.

Background: The purpose of this study was to investigate the effects of bone marrow-derived stem cells on consolidation period by using a new biomechanical testing method on sheep mandible model.

Methods: Eight sheep underwent bilateral mandibular osteotomies. After latency period, bone distraction was activated.

Evaluation of breast reduction surgery effect on body posture and gait pattern using three-dimensional gait analysis.

Unlabelled: Breast hypertrophy is a significant health burden with symptoms of back and shoulder pain, intertrigo, and shoulder grooving from the bra straps. Women often rely on surgery to relieve these symptoms, and they are mostly satisfied with the results. The satisfaction from surgery usually is evaluated by subjective measures.

One-staged silicone implant breast reconstruction following bilateral nipple-sparing prophylactic mastectomy in patients at high-risk for breast cancer.

Background: There is little consensus regarding the indications, ideal incision, or reconstruction method for prophylactic mastectomy. In addition, the biggest limitation in studies that assess either patient satisfaction or operation results is the lack of group homogeneity.

Methods: In our center, 21 bilateral prophylactic mastectomies, due to breasts at high risk for cancer, and simultaneous breast reconstruction using submuscular medium-height high-profile silicone implants were performed between 2008 and 2012.

Contour restoration of the forehead by lipofilling: our experience.

Background: A slightly convex forehead without wrinkles adds attractiveness to a woman's face and to the general perceived image. It is also important for the balance and harmony of the face, especially in the lateral and oblique views. We used a new fat injection technique for correction of the contours of the forehead.

Free fibular flap vascular pedicle calcification.

Osseous free flaps are major reconstruction choices of the segmental mandibular defects. However, etiology of defects in mandible is variable; tumor-related surgery, trauma, radiation, or congenital anomalies are the most common causes. The advent of microvascular surgery has achieved the reconstruction of complex mandibular defects.

Cerium nitrate treatment prevents progressive tissue necrosis in the zone of stasis following burn.

Cerium nitrate (CN) was used as a topical antiseptic agent for the treatment of burn wounds and found to reduce the number of anticipated death in burn. This decreased burn related mortality cannot be explained by the control of wound infection alone. In the studies performed to elucidate the unexplained effects of CN treatment, it was shown that CN treatment reduced the alarm cytokine levels, decreased leukocyte activation, reduced macromolecular leakage and finally burn edema formation.

Total lower eyelid reconstruction with superficial temporal fascia flap and porous polyethylene implant: a case report.

Total reconstruction of the eyelid after serious periorbital injury is a challenging procedure for plastic and reconstructive surgery. Although several methods have been used for reconstructing the eyelids, such as advancement flap with fascia lata sling, island mucochrondrocutaneous flap, prefabricated temporal island flap, porous polyethylene and superficial temporal fascia flap, creating a supportive eyelid for housing an artificial eye without complication is still an ongoing problem. In the case presented, superficial temporal fascia flap with porous polyethylene was used for the reconstruction of total lower eyelid.

A new perforator flap from distal medial arm: a cadaveric study.

Background: In this study, a new fasciocutaneous perforator flap raised from inner medial surface of the distal medial arm has been described for soft tissue coverage.

Methods: The blood supply of this flap comes directly from the distal brachial artery. Fourteen limbs of 7 formalinized cadavers were dissected to study the origin and the course of perforator vessel.

Trifocal biplanar distraction-compression osteosynthesis: a new method for three-dimensional reconstruction of the calvarial defects.

Surgical treatment of calvarial defects and deformities has some drawbacks such as donor site morbidity or use of allogenic materials. In this experimental study, we presented a three-dimensional reconstruction of cranial defects by using trifocal biplanar distraction-compression osteosynthesis (TBDCO).Eight sheep with a mean age of 2 years were divided into 2 groups as the control (n = 3) and distraction groups (n = 5).

Pain relief during dressing changes of major adult burns: ideal analgesic combination with ketamine.

Pain management during burn dressing changes is a critical part of treatment in acute burn injuries. Although several treatment options have been suggested, it is still a challenge in a clinical setting. This study is aimed at finding out an ideal analgesic, sedative and/or anxiolytic combination that would minimise the unwanted effects of ketamine.

Saving the zone of stasis in burns with activated protein C: an experimental study in rats.

Salvage of the zone of stasis is a major subject of focus in burn research. Use of various antithrombotic, anti-inflammatory and antioxidant drugs have been studied experimentally, with reports of favourable results; however, none became popular in clinical practice. Activated protein C (APC) is a well-known physiologic anticoagulant.

The crooked nose: the use of medial iliac crest bone graft as a supporting framework.

The crooked nose is one that the vertical axis of the nose inclines from the midline. As a rule, a major septal deformity almost always accompanies to impairing breathing and aesthetic appearance. To achieve total straightening of the crooked nose, the septum must become the target of the treatment.

A new finger fillet design from an index finger with severe postburn flexion deformity.

Treatment of postburn deformities of the hand is a real challenge to reconstructive surgeons. A functional reconstruction was achieved with two sensate island flaps on two discrete pedicles in a case with severe postburn deformity involving both thumb and index finger. Surgical treatment was based on amputation of the second ray and reconstruction of the thumb with the flaps derived from the discarded index finger.

Contour restoration of the secondary deformities of zygomaticoorbital fractures with porous polyethylene implant.

Inappropriate treatment or untreated fractures of the zygomaticoorbital area results in secondary deformities such as loss of malar projection, enophthalmos, and dystopia. Secondary deformities can be corrected with osteotomies, contour restoration, or a combination of both. Contour restoration can be performed with using onlay grafting with autogenous material or alloplastic implants.