Publications by authors named "Selanere L Mangala"

The purpose of this study was to investigate the antitumor effects of a combination of metronomic doses of a novel delivery vehicle, PLGA-PRINT nanoparticles containing docetaxel, and antiangiogenic mEZH2 siRNA incorporated into chitosan nanoparticles. In vivo dose-finding studies and therapeutic experiments were conducted in well-established orthotopic mouse models of epithelial ovarian cancer. Antitumor effects were determined on the basis of reduction in mean tumor weight and number of metastatic tumor nodules in the animals.

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Four sites of the non-homologous region (coding amino acid residues of 347, 421, 466 and 533) of a gene were randomly selected for splitting to investigate the function of beta-glucosidase from Agrobacterium tumefaciens in the co-refolding of peptides into the catalytically active enzyme. As a result of gene splitting, four N- and C-terminal domain peptides were obtained as insoluble inclusion bodies. No catalytic activity was observed when these fragments refolded individually.

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Article Synopsis
  • A carbohydrate-binding module (CBM2b) from Streptomyces thermoviolaceus was fused with a thermostable xylanase (XynB) from Thermotoga maritima to create a new enzyme called XynB-CBM2b.
  • The chimeric enzyme was purified and showed stability at high temperatures (up to 90 degrees C) and a similar pH-activity profile to XynB.
  • XynB-CBM2b was effective in binding to insoluble xylan sources and demonstrated slightly improved activity against soluble xylan compared to the original XynB enzyme.
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