Applications of Next-Generation Sequencing Technologies and Statistical Tools in Identifying Pathways and Biomarkers for Heat Tolerance in Livestock.

The climate change-associated abnormal weather patterns negatively influences the productivity and performance of farm animals. Heat stress is the major detrimental factor hampering production, causing substantial economic loss to the livestock industry. Therefore, it is important to identify heat-tolerant breeds that can survive and produce optimally in any given environment.

Climate Resilience in Farm Animals: Transcriptomics-Based Alterations in Differentially Expressed Genes and Stress Pathways.

The livestock sector, essential for maintaining food supply and security, encounters numerous obstacles as a result of climate change. Rising global populations exacerbate competition for natural resources, affecting feed quality and availability, heightening livestock disease risks, increasing heat stress, and contributing to biodiversity loss. Although various management and dietary interventions exist to alleviate these impacts, they often offer only short-lived solutions.

Applications of Artificial Intelligence for Heat Stress Management in Ruminant Livestock.

Heat stress impacts ruminant livestock production on varied levels in this alarming climate breakdown scenario. The drastic effects of the global climate change-associated heat stress in ruminant livestock demands constructive evaluation of animal performance bordering on effective monitoring systems. In this climate-smart digital age, adoption of advanced and developing Artificial Intelligence (AI) technologies is gaining traction for efficient heat stress management.

Comparative assessment of climate resilient potential in four poultry genotypes reared in hot-humid tropical environment: a preliminary evaluation.

The general objective of this study is to comparatively assess the climate-resilient potential of four different poultry genotypes-Giriraja (n = 8), Country chicken (n = 8), Naked neck (n = 8), and Kadaknath (n = 8)-reared in a hot-humid tropical environment. Birds from all genotypes had ad libitum access to feed and water and were exposed to identical environmental temperatures in the experimental shed. Diurnal meteorological data were recorded inside and outside the shed daily.

Do newborn lambs with black and white hair-coats in an equatorial semi-arid environment maintain homeothermy?

We assessed newborn lambs from two hair-coat sheep breeds, the black Santa Ines (n = 29) and white Dorper (n = 26), to determine how they behaviourally and physiologically respond to the prevailing thermal conditions in an equatorial semi-arid environment. Measurements of hair-coat surface temperature, rectal temperature and the lambs' exposure to sun were recorded across the first 24 h of life every hour, after the lambs had received colostrum. Lambs and ewes were kept in a lambing pen and could freely move between a shaded area or be exposed to sun.

Comparative assessment of growth performance, heat resistance and carcass traits in four poultry genotypes reared in hot-humid tropical environment.

This study investigated the impact of heat stress on growth and carcass traits in four poultry genotypes-Giriraja, Country chicken, Naked Neck and Kadaknath reared in a hot and humid tropical environment. Birds from all genotypes had ad libitum access to feed and water while being challenged with consistently high environmental temperatures in the experimental shed. Daily diurnal meteorological data were recorded inside and outside the shed.

Unraveling the Genomic Association for Milk Production Traits and Signatures of Selection of Cattle in a Harsh Tropical Environment.

A study was designed to identify the genomic regions associated with milk production traits in a dairy cattle population reared by smallholder farmers in the harsh and challenging tropical savanna climate of Bengaluru, India. This study is a first-of-its-kind attempt to identify the selection sweeps for the dairy cattle breeds reared in such an environment. Two hundred forty lactating dairy cows reared by 68 farmers across the rural-urban transiting regions of Bengaluru were selected for this study.

Comparing the responses of grain-fed feedlot cattle under moderate heat load and during subsequent recovery with those of feed-restricted thermoneutral counterparts: blood cells and inflammatory markers.

Given the climate projections for livestock rearing regions globally, understanding the inflammatory status of livestock under various heat loads will be informative to animal welfare and management. A survey of plasma inflammatory markers was conducted, and blood leucocyte counts followed to investigate the capacity of the ~ 500 kg grain fed Black Angus steer to respond to and recover from a moderate heat load challenge. Two sequential cohorts of 12 steers were housed in climate-controlled rooms (CCR) for 18 days.

Selective Sweeps in Cattle Genomes in Response to the Influence of Urbanization and Environmental Contamination.

A genomic study was conducted to identify the effects of urbanization and environmental contaminants with heavy metals on selection footprints in dairy cattle populations reared in the megacity of Bengaluru, South India. Dairy cattle reared along the rural-urban interface of Bengaluru with/without access to roughage from public lakeshores were selected. The genotyped animals were subjected to the cross-population-extended haplotype homozygosity (XP-EHH) methodology to infer selection sweeps caused by urbanization (rural, mixed, and urban) and environmental contamination with cadmium and lead.

Stress Factors and Their Effects on Productivity in Sheep.

Products obtained from sheep have an economically important place in the world. Their adaptability to different climatic conditions, their ease of care and feeding, their high utilization of poor pasture areas with low yield and quality, the ease of flock management, their high twinning rate, and their short intergenerational period are some of the advantages of sheep production. Sheep production has the ability to adapt better to environmental stress factors, as can be understood from the presence of sheep in different geographical regions at a global level.

The autumn low milk yield syndrome in Brown Swiss cows in continental climates: hypotheses and facts.

Extensive research has been conducted globally on the impact of heat stress (HS) on animal health and milk production in dairy cows. In this article, we examine the possible reasons for the decrease in milk production in Brown Swiss (BS) cows during the autumn season, known as the autumn low milk yield syndrome (ALMYS). This condition has been extensively studied in high-yielding Holstein Friesian (HF) cattle and has also been observed in BS cows with a daily milk yield of around 30 kg.

Comparative assessment of growth performance of indigenous and cross-bred calves subjected to combined stressors (heat and nutritional).

This study evaluated the impact of combined stressors (heat and nutritional stresses) on the growth and adaptive capability of Sahiwal (SW) and Karan Fries (KF) calves during the summer season. Calves in each breed were randomly divided into four groups. In SW breed the groupings were as follows: SWC (n = 4; Sahiwal Control); SWHS (n = 4; Sahiwal Heat Stress); SWNS (n = 4; Sahiwal Nutritional Stress) and SWCS (n = 4; Sahiwal Combined Stresses).

Seasonal, physiological and bacteriological risk factors for subclinical mastitis in dairy cows maintained under different farming conditions.

Subclinical mastitis (SCM) is a major health problem of dairy animals in India and across the globe. An identification of potential risk factors of SCM can help for efficient udder health management in dairy animals. In this study, apparently healthy cows (HF crossbred: = 45; Deoni: = 43) were screened for SCM during different seasons through milk somatic cell count (SCC: reference test using 200 × 10 cells/ml as cut off value), California mastitis test (CMT) and differential electrical conductivity (DEC) test at an organized research farm.

Bibliographic mapping for heat tolerance in pigs and poultry.

Heat tolerance, especially under climate change scenarios, plays an increasingly import factor in pig and chicken production. We therefore evaluated bibliographic mapping of citation, co-occurrence of keywords, co-citation and bibliographic coupling for heat tolerance, and these species. Data was obtained from Scopus (Elsevier) and analysed in Vosviewer.

Novel Insights to Assess Climate Resilience in Goats Using a Holistic Approach of Skin-Based Advanced NGS Technologies.

A novel study was conducted to elucidate heat-stress responses on a number of hair- and skin-based traits in two indigenous goat breeds using a holistic approach that considered a number of phenotypic and genomic variables. The two goat breeds, Kanni Aadu and Kodi Aadu, were subjected to a simulated heat-stress study using the climate chambers. Four groups consisting of six goats each (KAC, Kaani Aadu control; KAH, Kanni Aadu heat stress; KOC, Kodi Aadu control; and KOH, Kodi Aadu heat stress) were considered for the study.

Molecular, Physiological and Hematological Responses of Crossbred Dairy Cattle in a Tropical Savanna Climate.

A comprehensive study was conducted to assess the effects of seasonal transition and temperature humidity index (THI) on the adaptive responses in crossbred dairy cows reared in a tropical savanna region. A total of 40 lactating dairy cattle reared by small-scale dairy farmers in Bengaluru, India, were selected for this study. The research period comprised the transitioning season of summer to monsoon, wherein all traits were recorded at two points, one representing late summer (June) and the other early monsoon (July).

Heat tolerance, thermal equilibrium and environmental management strategies for dairy cows living in intertropical regions.

This review makes an attempt to characterize the physical attributes of heat tolerance, thermal equilibrium and thermal stress thresholds for dairy cows living in tropical environments, with a particular emphasis on pasture-based systems. Under such circumstances, the radiant heat load is the principal climatic factor that determines rates of heat and mass exchanges between cows and the environment. This fact may explain why simple mechanistic models based on air temperature and humidity are not adequately predicting thermal stress thresholds for cattle in tropical regions.

Diversity of rumen microbiota using metagenome sequencing and methane yield in Indian sheep fed on straw and concentrate diet.

An study aiming to investigate the rumen methanogens community structure was conducted in sheep fed on straw and concentrate diet. The ruminal fluid samples were collected and processed for unravelling the rumen microbiota and methanogens diversity. Further, the daily enteric methane emission and methane yield was also quantified using the SF tracer technique.

Analysis of carcass traits and quantitative expression patterns of different meat quality governing genes during heat stress exposure in indigenous goats.

A study was conducted to assess the impact of heat stress on various carcass traits, meat quality variables and gene expression patterns which governs meat quality in indigenous female Kodi Aadu breed. The study was conducted for 45 days in climate chamber with 12 animals randomly allocated into two groups of six animals each, KC (n = 6; Female; Control), KHS (n = 6; Female; heat stress). Majority of the major carcass traits and meat quality variables remained intact between KC and KHS groups.

Changes in the Spectrum of Free Fatty Acids in Blood Serum of Dairy Cows during a Prolonged Summer Heat Wave.

This experiment was conducted to study the effect of a prolonged hot period on the fatty acid (FA) composition in blood serum of dairy cows. Eighteen multiparous Holstein cows were randomly assigned to the hyperthermia group (HYP, = 8) in August (summer season) and the control group (CON, = 10) in October (autumn season). Blood from animals of the HYP group was collected in one heat wave, which was preceded by a long period of heat stress (HS, temperature-humidity index (THI ≥ 72)).

Increasing importance of heat stress for cattle farming under future global climate scenarios.

In the last decades, livestock species have been severely affected by heat stress because of increasing temperatures, which has threatened animal welfare and decreased production. Based on thermal comfort indices and ensemble climate projections, we analyzed the current and future global spatiotemporal patterns of the heat exposure of cattle in 10 agroclimatic zones. The results show that ~7% of the global cattle population is currently exposed to dangerous heat conditions.

Comparative Expression Profiling and Sequence Characterization of ATP1A1 Gene Associated with Heat Tolerance in Tropically Adapted Cattle.

Climate change is an imminent threat to livestock production. One adaptation strategy is selection for heat tolerance. While it is established that the gene and its product play an important role in the response to many stressors, there has been no attempt to characterize the sequence or to perform expression profiling of the gene in production animals.