Publications by authors named "Seita Doi"
To elucidate S100 protein-mediated signaling pathways, we attempted to identify novel binding partners for S100A2 by screening protein arrays carrying 19,676 recombinant glutathione S-transferase (GST)-fused human proteins with biotinylated S100A2. Among newly discovered putative S100A2 interactants, including TMLHE, TRH, RPL36, MRPS34, CDR2L, OIP5, and MED29, we identified and characterized the tubulin polymerization-promoting protein (TPPP) as a novel S100A2-binding protein. We confirmed the interaction of TPPP with Ca/S100A2 by multiple independent methods, including the protein array method, S100A2 overlay, and pulldown assay in vitro and in transfected COS-7 cells.
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- Identified HMG20A as a new binding partner for the S100A6 protein during protein-protein interaction screening using human protein arrays.
- Confirmed the interaction's dependency on calcium through various assays, including co-immunoprecipitation in cell lines.
- Demonstrated that HMG20A interacts specifically with certain S100 proteins, and highlighted the importance of a specific C-terminal region of HMG20A for binding, suggesting potential roles in calcium signaling and neural differentiation.