Evaluation of the Rotating-Frame Relaxation () Filter and Its Application in Metabolomics as an Alternative to the Transverse Relaxation () Filter.

Nuclear magnetic resonance (NMR)-based metabolomic studies commonly involve the use of filter pulse sequences to eliminate or attenuate the broad signals from large molecules and improve spectral resolution. In this paper, we demonstrate that the filter-based pulse sequence represents an interesting alternative because it allows the stability and the reproducibility needed for statistical analysis. The integrity of the samples and the stability of the instruments were assessed for different filter durations and amplitudes.