BMC Complement Med Ther
August 2024
The emergence of antimicrobial resistance has led to an urgent need for novel antimicrobial drugs. This study aimed to determine the antioxidant and antimicrobial potentials in silico and in vitro of Pandanus amaryllifolius Roxb. ethanolic extract.
View Article and Find Full Text PDFThe most common gram-negative, , and gram-positive bacteria, spp., have evolved different mechanisms that have caused the emergence of multi-drug resistance. As a result, drugs that block the bacterial growth cycle are needed.
View Article and Find Full Text PDFBackground: Cymbopogon is a member of the family Poaceae and has been explored for its phytochemicals and bioactivities. Although the antimicrobial activities of Cymbopogon spp. extracts have been extensively studied, comprehensive analyses are required to identify promising compounds for the treatment of antimicrobial resistance.
View Article and Find Full Text PDFLiamocins, a group of high-density glycolipids, are only produced by certain strains of the yeast-like fungi in the genus Aureobasidium. Until now, few studies have focused on the surfactant properties of liamocins produced from the highly diverse tropical strains of Aureobasidium. Therefore, the aims of this research were to screen the liamocin production from tropical strains of Aureobasidium spp.
View Article and Find Full Text PDFIn an attempt to enhance the value of sugarcane leaf, xylan was extracted and used for xylooligosaccharide (XO) production via enzymatic hydrolysis using xylanase from the black yeast . The xylan was extracted from sugarcane leaf using alkali extraction according to the response surface methodology. The highest xylan yield (99.
View Article and Find Full Text PDFFungal polysaccharides have garnered interest due to their biological activities in terms of anticancer properties and antioxidant activity. The present study aimed to evaluate the anticancer properties and antioxidant activity of a newly isolated white-rot fungus, CU07 from Thailand. Crude polysaccharides (CTPPs) were extracted from mycelia using hot water.
View Article and Find Full Text PDFTreatment with extracts from whole herbs has been reported to synergistically enhance the anticancer activities of therapeutic agents in recent studies. The present study evaluated the antioxidant and anticancer activities of () and () crude extracts individually and in combination. was extracted using ethanol, whereas was extracted using hot water.
View Article and Find Full Text PDFNatural products play an essential role in new drug discovery. In the present study, we determined the anti-SARS-CoV-2 (severe acute respiratory syndrome-related coronavirus-2), antioxidant, antiplasmodial, and antimicrobial activities of Kurz. heartwood and structurally characterized the bioactive compounds.
View Article and Find Full Text PDF3 Biotech
September 2022
Unlabelled: The maximum yield of the crude mannanase from NRRL 58524 was 8.42 ± 0.18 U mL when cultured for 72 h in the optimized medium containing 3% (w v) defatted spent coffee grounds (SCG) and 0.
View Article and Find Full Text PDFMalaria is still a global health problem. Plasmodium is a single-cell protozoan parasite that causes malaria and is transmitted to humans through the female Anopheles mosquito. The previous study showed that Sonchus arvensis L.
View Article and Find Full Text PDFOil palm empty fruit bunch (EFB) pulp with the highest cellulose content of 83.42% was obtained from an optimized process of acid pretreatment (0.5% v/v HSO), alkaline extraction (15% w/w NaOH), and hydrogen peroxide bleaching (10% w/v HO), respectively.
View Article and Find Full Text PDFThe newly isolated PBURU 12 demonstrated a high tolerance and potential for the degradation of phenanthrene. The fungal isolate was able to tolerate 100 ppm of phenanthrene with 45% relative growth. The crude laccase produced by PBURU 12 was able to degrade phenanthrene by up to 98% within 24 h.
View Article and Find Full Text PDFAmong 23 isolates of cutinase-producing fungi from Thailand, one strain of PBURU-T5 exhibited the greatest cutinase activity (3.36 ± 0.12 U ml) against -nitrophenyl butyrate.
View Article and Find Full Text PDFSurfactants are used to stabilize nanoemulsions by protecting their physical stability and preventing deterioration of the entrapped bioactive during processing and storage. The effect of surfactant concentration on physical-chemical properties of nanoemulsions with entrapped curcumin, relevant to commercial applications, was addressed in this research. Furthermore, the functionality of nanoemulsified curcumin in terms of lipid oxidation inhibition was determined.
View Article and Find Full Text PDFA cellulase from the extreme obligate halophilic fungus, Aspergillus flavus, isolated from a man-made solar saltern in Phetchaburi, Thailand, was purified by ammonium sulfate precipitation and using Sephadex G-100 gel filtration column chromatography. The cellulase was found to be approximately 55 kDa by SDS-PAGE. Using CMC as a substrate, the specific activity of the cellulase was 62.
View Article and Find Full Text PDFBackground/aim: Oxidative stress caused by the production of excessive cellular reactive oxygen species (ROS) and high levels of nitric oxide contribute to several human pathologies. This study aimed to examine the anti-oxidant effects of fusigen, a compound produced from Aureobasidium melanogenum.
Materials And Methods: Extracts of A.
The maximum yield of xylanase from PBUAP46 was 5.19 ± 0.08 U ml when cultured in a production medium containing 3.
View Article and Find Full Text PDFDifferent strains of the saprophytic yeast-like fungus Aureobasidium pullulans (Ascomycota: Dothideales) exhibit different biochemical characteristics, while their ubiquitous occurrence across diverse habitats and environmental conditions makes them an easily accessible source for biotechnological exploitation. They are useful in agricultural and industrial applications. Their antagonistic activities against postharvest pathogens make them suitable bioagents for the postharvest preservation of fruits and vegetables, while they possess antimicrobial activities against bacteria and fungi.
View Article and Find Full Text PDFFrom three cell-associated β-xylosidases produced by Aureobasidium pullulans CBS 135684, the principal enzyme was enriched to apparent homogeneity and found to be active at high temperatures (60-70 °C) over a pH range of 5-9 with a specific activity of 163.3 units (U) mg. The enzyme was thermostable, retaining over 80% of its initial activity after a 12-h incubation at 60 °C, with half-lives of 38, 22, and 10 h at 60, 65, and 70 °C, respectively.
View Article and Find Full Text PDFPostharvest herbal product contamination with mycotoxins and mycotoxin-producing fungi represents a potentially carcinogenic hazard. Aspergillus flavus is a major cause of this issue. Available mold detection methods are PCR-based and rely heavily on laboratories; thus, they are unsuitable for on-site monitoring.
View Article and Find Full Text PDFAn extracellular α-amylase from the obligate halophilic Aspergillus penicillioides TISTR3639 strain was produced and enriched to apparent homogeneity by ammonium sulfate precipitation and Sephadex G100 gel filtration column chromatography. The mass of the purified amylase was estimated to be 42 kDa by SDS-PAGE. With soluble starch as the substrate it had a specific activity of 118.
View Article and Find Full Text PDFAn obligate halophilic Aspergillus gracilis which was isolated from a hypersaline man-made saltern from Thailand was screened for its potential of producing extracellular α -amylase in the previous studies. In this study the α -amylase was extracted and purified by the help of column chromatography using Sephadex G-100 column. Presence of amylase was verified by SDS-PAGE analysis, showing a single band of approximately 35 kDa.
View Article and Find Full Text PDFA water-soluble chitosan with improved mucoadhesion was prepared by modifying 19.4% of the amine groups of chitosan to trimethylammonium and conjugation of gluconolactone (GLU) and homocysteine thiolactone (HT) onto the remaining amine groups of the chitosan backbone. The derived trimethyl-gluconate-HT-chitosan (TM-GN-HT-chitosan) was confirmed by Fourier Transform Infrared spectroscopy, NMR and thermogravimetric analysis.
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