Enrichment of spermatogonial stem cells from long-term cultured human testicular cells.

Objective: To evaluate the degree of enrichment of spermatogonial stem cells (SSCs) from human testicular cell cultures by ITGA6+, HLA-/ITGA6+, GPR125+, and HLA-/GPR125+ magnetic-assisted cell sorting (MACS).

Design: Experimental basic science study.

Setting: Reproductive biology laboratory.

Transient receptor potential vanilloid receptor-1 confers heat resistance to male germ cells.

Testicular hyperthermia in mice lacking transient receptor potential vanilloid receptor-1 results in a much more rapid and massive germ cell depletion from the seminiferous tubules than in wild-type animals, indicating that this receptor protects germ cells against heat stress.