Green HPLC Method for Simultaneous Analysis of Three Natural Antioxidants by Analytical Quality by Design.

Background: Glutathione, silybin, and curcumin are well-known potential antioxidants that are recommended as adjuvant therapy in cancer treatment.

Objective: Based on the principles of Analytical Quality by Design (AQbD) and green analytical chemistry, a simple, robust, and environmentally benign HPLC method for the simultaneous estimation of glutathione, silybin, and curcumin in bulk and formulation was performed.

Method: Elution was achieved by an Agilent Eclipse XDB C18 (150 mm × 4.