Non-industrial production of therapeutic lentiviral vectors: How to provide vectors to academic CAR-T.

Bringing effective cancer therapy in the form of chimeric antigen receptor technology to untapped markets faces numerous challenges, including a global shortage of therapeutic lentiviral or retroviral vectors on which all current clinical therapies using genetically modified T cells are based. Production of these lentiviral vectors in academic settings in principle opens the way to local production of therapeutic cells, which is the only economically viable approach to make this therapy available to patients in developing countries. The conditions for obtaining and concentrating lentiviral vectors have been optimized and described.

Treatment of atopic dermatitis with upadacitinib: adcare single center experience.

Introduction: The role of upadacitinib in the management of moderate to severe atopic dermatitis seems promising, but more data on its efficacy and safety are needed. This study endeavors to assess the practical impact and safety of upadacitinib in patients with moderate to severe atopic dermatitis. The study aims to evaluate the efficacy and safety of upadacitinib in the treatment of moderate to severe atopic dermatitis, focusing on analyzing patient responses to the treatment.

Multimodal OCT Control for Early Histological Signs of Vulvar Lichen Sclerosus Recurrence after Systemic PDT: Pilot Study.

Photodynamic therapy (PDT) is a modern treatment for severe or treatment-resistant vulvar lichen sclerosus (VLS). The chronic and recurrent nature of VLS requires control of recurrences at an early stage. In this paper, a non-invasive multimodal optical coherence tomography (OCT) method was used to control for early histological signs of VLS recurrence after systemic PDT using Photodithazine.

Non-neutralizing Antibodies Directed at Conservative Influenza Antigens.

At the moment, developing new broad-spectrum influenza vaccines which would help avoid annual changes in a vaccine's strain set is urgency. In addition, developing new vaccines based on highly conserved influenza virus proteins could allow us to better prepare for potential pandemics and significantly reduce the damage they cause. Evaluation of the humoral response to vaccine administration is a key aspect of the characterization of the effectiveness of influenza vaccines.

Optical coherence angiography for pre-treatment assessment and treatment monitoring following photodynamic therapy: a basal cell carcinoma patient study.

Microvascular networks of human basal cell carcinomas (BCC) and surrounding skin were assessed with optical coherence angiography (OCA) in conjunction with photodynamic therapy (PDT). OCA images were collected and analyzed in 31 lesions pre-treatment, and immediately/24 hours/3-12 months post-treatment. Pre-treatment OCA enabled differentiation between prevalent subtypes of BCC (nodular and superficial) and nodular-with-necrotic-core BCC subtypes with a diagnostic accuracy of 78%; this can facilitate more accurate biopsy reducing sampling error and better therapy regimen selection.

Vaccination potential of B and T epitope-enriched NP and M2 against Influenza A viruses from different clades and hosts.

To avoid outbreaks of influenza virus epidemics and pandemics among human populations, modern medicine requires the development of new universal vaccines that are able to provide protection from a wide range of influenza A virus strains. In the course of development of a universal vaccine, it is necessary to consider that immunity must be generated even against viruses from different hosts because new human epidemic virus strains have their origins in viruses of birds and other animals. We have enriched conserved viral proteins-nucleoprotein (NP) and matrix protein 2 (M2)-by B and T-cell epitopes not only human origin but also swine and avian origin.

[An effect of cerebral hemodynamics in patients with cerebrovascular disorders on the in-hospital transportation].

In-hospital transportation of 76 patients with cerebrovascular disorders, including those in the first postoperative day, was performed. Two cerebral hemodynamic measurements were carried out five minutes before and immediately after transportation using transcranial Doppler sonography. Significant changes in cerebral blood flow in the operated patients were shown.

NMR study of phthalide-type poly(phenylene)s. Symmetry and additivity.

All H and C NMR signals of the four poly(phenylenephthalides): polydiphenylenephthalide (P(DPh)-1), polyterphenylenephthalide (P(TPh)-2) and two sequentially ordered polymers with different ratios of alternating diphenylenephthalide and terphenylenephthalide units (P(DTPh)-3, P(DDTPh)-4) were assigned unequivocally with two-dimensional NMR techniques ( H- H COSY and NOESY; H- C HSQC and HMBC). There are four types of polyphenylene fragments: not symmetrical end, symmetrical inner, symmetrical pre-end and formally symmetric pre-end. The equivalent carbon atoms in these fragments have different chemical shifts.

[Effects of body positioning in patients with non-traumatic intracranial hemorrhages].

The article presents an analysis of different methods of positioning patients with acute cerebral pathology. Changing of 'head-of-the bed' position is a routine and simple method of correction of intracranial hypertension. For the majority of patients with cerebral damage, regardless of the etiological factor, 15-30° 'head - of - the bed' position is preferable.

[NUTRITIONAL STATUS IN THE EXPERIMENT WITH 105-DAY ISOLATION AS THE FIRST PHASE OF PROJECT MARS-500].

In a 105-day experiment simulating crew life in a space exploration vehicle, shifts in the nutritional status were assessed in 6 test subjects differing in the body mass index, basal metabolism, attitude to the diet, physical exercise and workload. Results of the investigation showed that because of the hard physical work the nutritional status of test subjects was described by more intensive basal metabolism, elevated metabolism of carbohydrates and lipids, and their increased mobilization from depots. Food ration, though it was sufficient to sustain health and fairly high performance, failed to meet fully individual taste preferences and energy needs for physical activities.

[Evaluation of the liver functional changes due to modeling the hemodynamic effects of microgravity in bed rest studies].

Ultrasonic investigations of 8 male volunteers for bed rest at -15 degrees showed plethora in peritoneal veins and slow blood flow through the liver with no effect on biochemical parameters. Breath test with 100 mg of 13C-methacetine (MBT) pointed to diminution of the metabolic capacity and decline of the hepatic detoxification activity due to the hemodynamic effects. MBT can be used as a nonintrusive method for diagnosing functional changes in the liver induced by hemodynamic developments in microgravity and other situations triggering similar hemodynamic mechanisms.

[Functional activity of the liver in immersion and effects of the countermeasures].

Two groups of male volunteers for 4-day dry immersion with and w/o countermeasures (support load imitator (SLI) or high-frequency electrostimulator) underwent ultrasonic investigation (USI) of the liver, gastroduodenal organs and vessels, and blood biochemical analysis. Two other groups of volunteers performed the 13C-methacetin breath test (13C-MBT) to study the effects of immersion and SLI on the liver detox activity and metabolic capacity. In immersion, USI diagnosed slowdown of blood flow along the hepatic vein and signs of plethora in the abdominal venous system.

[Construction of recombinant adenoviral vector expressing genes of the conservative influenza proteins M2 and nucleoprotein].

Influenza is a highly contagious and one of the most massive infection diseases. General epidemiological significance has a strain, which belongs to subtype A. A high degree of genetic variety leads to the permanent changes in the antigenic structure of the influenza virus.

[Influence of body position on intracranial pressure and cerebral hemodynamic in non-traumatic intracranial hemorrhages].

Objective: Patient's body position is one of the most important principles of intensive therapy of intracranial hypertension syndrome. It is considered that the provision of the head end of a bed of 15-30 degrees is preferable for the majority of patients with cerebral pathology. We studied hemodynamic parameters at various tilt angles of the body in patients with non-traumatic intracranial hemorrhages.

13C NMR spectroscopy of copoly(arylenephthalide) derivatives with diphenyloxide and terphenyl fragments in the main chain.

(1) H and (13) С NMR spectral assignments have been provided for low-molecular reference monomers, poly(diphenyleneoxidephthalide) and periodic copoly(arylenephthalide) derivatives such as AB, ABB, and ABBB (where A = terphenylenephthalide and B = diphenyleneoxidephthalide) using (1) H-(1) H COSY, (1) H-(13) C HSQC and HMBC NMR techniques. Distinctive (13) C NMR chemical shifts of a main chain have been observed containing fragments of similar structures and lateral phthalate groups being part of various diads.

Recombinant influenza vaccines.

This review covers the problems encountered in the construction and production of new recombinant influenza vaccines. New approaches to the development of influenza vaccines are investigated; they include reverse genetics methods, production of virus-like particles, and DNA- and viral vector-based vaccines. Such approaches as the delivery of foreign genes by DNA- and viral vector-based vaccines can preserve the native structure of antigens.

[Hybrid methods in the treatment of heart disease in elderly and senile patients].

Hybrid technologies are becoming increasingly strong position in modern cardiac surgery. New standards and expanding the indications for use of these techniques are delivered. The advantages of hybrid interventions are to a substantial reduction in trauma patients during surgery, reducing the risk of both intra- and postoperative complications, death, the most important for the elderly and senile age.

[The role of vasoactive peptid in lower limbs chronic arterial insufficiency treatment].

Unlabelled: Atherosclerosis has lead to lower limbs chronic occlusive arterial disease in 80-90 % of cases. The progress of atherosclerosis is vascular wall self-regulation process violation, and its correction can be made by completion of missing endogenous peptides.

Objective: assessment of the possibility to use the vasoactive peptid of Vezugen as monotherapy in patients underwent surgery for chronic lower limbs ischemia.

Passive immunization with a recombinant adenovirus expressing an HA (H5)-specific single-domain antibody protects mice from lethal influenza infection.

One effective method for the prevention and treatment of influenza infection is passive immunization. In our study, we examined the feasibility of creating an antibody-based preparation with a prolonged protective effect against influenza virus. Single-domain antibodies (sdAbs) specific for influenza virus hemagglutinin were generated.

Formatted single-domain antibodies can protect mice against infection with influenza virus (H5N2).

This work continues a series of recently published studies that employ recombinant single-domain antibody (sdAb, or nanobody®) generation technologies to battle viruses by a passive immunization approach. As a proof of principle, we describe a modified technique to efficiently generate protective molecules against a particular strain of influenza virus within a reasonably short period of time. This approach starts with the immunization of a camel (Camelus bactrianus) with the specified antigen-enriched material presented in as natural a form as possible.

[Investigation of the evacuatory function of the gastrointestinal tract in 5-day dry immersion].

Six male volunteers for dry immersion (DI) modeling the microgravity effects on human organism were subjects in the GI evacuatory function investigation. The investigation was aimed to evaluate liquid food evacuation from the stomach (13C-acetate test) and time of chymus orocecal transit (H2-inulin test). The 13C-acetate test did not reveal changes in stomach evacuatory activity after 4 days in DI.

[Protective properties of candidate genetically engineered vaccines against avian influenza viruses constructed on the basis of recombinant adenoviral vectors].

Aim: To design and study the properties of candidate vaccines against avian influenza based on recombinant adenoviral vectors expressing H5 hemagglutinin.

Materials And Methods: Recombinant adenoviral vectors were constructed as described in "Stratagene" (Ad Easy Adenoviral Vector System). For immunization of animals, recombinant candidate vaccines were administered intranasally twice.

Induction of a Protective Heterosubtypic Immune Response Against the Influenza Virus by using Recombinant Adenoviral Vectors Expressing Hemagglutinin of the Influenza H5 Virus.

Influenza viruses are characterized by a high degree of antigenic variability, which causes the annual emergence of flu epidemics and irregularly timed pandemics caused by viruses with new antigenic and biological traits. Novel approaches to vaccination can help circumvent this problem. One of these new methods incorporates genetic vaccines based on adenoviral vectors.

[Digestive system functioning during simulation of the microgravity effects on humans by immersion].

Functioning of the digestive system was investigated in 10 volunteers for 7-day dry immersion. The experimental conditions were found to raise secretory activities of the stomach, pancreas and liver, and to increase spectral indices of the GI electrical activity on the background of a higher than usual insular secretion and lowered gastrin secretion. The elevated GI electrical activities and changes in their ratios were a fallout of the increased gastric secretion and elevated intestines tone in fasting test-subjects and displayed a close similarity to the changes induced by caffeine stimulation, long-term bed rest or space flight.