Cross-linked α-galactosidase aggregates: optimization, characterization and application in the hydrolysis of raffinose-type oligosaccharides in soymilk.

Background: Cross-linked enzyme aggregates (CLEAs) of α-galactosidase, partially purified from maize (Zea mays) flour, were prepared. The impact of various parameters on enzyme activity was examined to optimize the immobilization procedure. Biochemical characterization of the free and immobilized enzyme was carried out.

Molecularly imprinted polymers for separation of various sugars from human urine.

Molecularly imprinted polymers were the new, simple and unexpensive materials that can be used in several clinical applications. Phenylboronic acid has been frequently used as functional monomer for the covalent imprinting of diols. In this study, the phenylboronic acid esters of fructose, galactose, glucose and raffinose were synthesized and then used as template analytes.

Immobilization and stabilization of α-galactosidase on Sepabeads EC-EA and EC-HA.

α-Galactosidase from tomato has been immobilized on Sepabead EC-EA and Sepabead EC-HA, which were activated with ethylendiamino and hexamethylenediamino groups, respectively. Two strategy was used for the covalent immobilization of α-galactosidase on the aminated Sepabeads: covalent immobilization of enzyme on glutaraldehyde activated support and cross-linking of the adsorbed enzymes on to the support with glutaraldehyde. By using these two methods, all the immobilized enzymes retained very high activity and the stability of the enzyme was also improved.

Efficient and rapid purification of lentil alpha-galactosidase by affinity precipitation with alginate.

Alpha-Galactosidase (alpha-D-galactoside galactohydrolase, EC 3.2.1.

Biosorption of chromium, cadmium, and cobalt from aqueous solution by immobilized living cells of Chryseomonas luteola TEM 05.

In this work, the potential use of the immobilized cells of Chryseomonas luteola TEM 05 for the removal of Cr(+6), Cd(+2) and Co(+2) ions from aqueous solutions was investigated. The living cells of C. luteola TEM 05 were firstly entrapped both in carrageenan and chitosan coated carrageenan gels and then used in biosoption of the metal ions in batch reactors at pH 6.

Immobilization of avocado phytase on epoxy-activated Sepabead EC-EP and its application in soymilk phytate hydrolysis.

There is a great demand for using phytases to reduce phytate content in animal feed stuffs and food for human consumption. Industrial application of phytase requires efficient methods to immobilize the enzyme, yielding a biocatalyst with high activity and stability compared to the free enzyme. In the present study, phytase was immobilized on Sepabead EC-EP and then used in the biodegradation of soymilk phytate.

Differentially displayed proteins as a tool for the development of type 2 diabetes.

Background: Type 2 diabetes is a complex disease that still requires a great deal of work to be carried out to understand the pathophysiology. Recently, researchers have focused on studying the organs and tissues known to be involved in the development of the type 2 phenotype using a proteomic approach. Little work has been reported on plasma of type 2 diabetics in whom the clinical status has been well characterized.

Noncovalently galactose imprinted polymer for the recognition of different saccharides.

Molecularly imprinted polymers (MIPs) represent a new class of materials possessing high selectivity and affinity for the target molecule. The main goal of this study was to prepare a galactose imprinted polymer and its potential application for the recognition of different saccharides. The selectivity of galactose imprinted polymer for several saccharides; glucose, mannose, fructose, maltose, lactose, sucrose and raffinose was investigated.

Purification of alpha-galactosidase by affinity precipitation with alginate.

Affinity precipitation is a technique which is known for over 20 years, but has recently received more attention due to the development of new materials for its implementation. It is a relatively simple, convenient, and reproducible technique that results in high target molecule recovery at high specificity. We describe, here, an efficient and rapid purification procedure for Vicia faba alpha-galactosidase (EC 3.

Studies on the applicability of alginate-entrapped Chryseomonas luteola TEM 05 for heavy metal biosorption.

Chryseomonas luteola TEM 05 cells were entrapped both in alginate and chitosan coated alginate beads. Biosorption of metal ions on alginate beads was investigated by using a batch stirred system at pH 6.0, 25 degrees C, in initial metal concentration of 1.

Comparative studies on the adsorption of Cr(VI) ions on to various sorbents.

The adsorption of Cr(VI) ions onto various sorbents (chitin, chitosan, ion exchangers; Purolite CT-275 (Purolite I), Purolite MN-500 (Purolite II) and Amberlite XAD-7) was investigated. Batch adsorption experiments were carried out as a function of pH, agitation period and concentration of Cr(VI) ions. The optimum pH for Cr(VI) adsorption was found as 3.

Immobilization of pancreatic lipase on chitin and chitosan.

In this study, porcine pancreatic lipase (EC 3.1.1.

Inhibition of alpha-glucosidase by aqueous extracts of some potent antidiabetic medicinal herbs.

Diabetes mellitus is one of the most prevalant diseases of adults. Agents with alpha-glucosidase inhibitory activity have been useful as oral hypoglycemic drugs for the control of hyperglycemia in patients with type 2; noninsulin-dependent, diabetes mellitus (NIDDM). Investigation of some medicinal herbs: Urtica dioica, Taraxacum officinale, Viscum album, and Myrtus communis with alpha-glucosidase inhibitor activity was conducted to identify a prophylactic effect for diabetes in vitro.

Immobilization of phospholipase A2 on porous glass and its application for lowering serum cholesterol concentration.

Phospholipase A2 (PLA2; EC 3.1.1.

Preparation and properties of alpha-galactosidase chemically attached to activated chitin.

alpha-Galactosidase (alpha-D-galactoside galactohydrolase, EC 3.2.1.

An enzyme electrode based on lipoxygenase immobilized in gelatin for selective determination of essential fatty acids.

An enzyme electrode for the specific determination of omega-3 and omega-6 fatty acids from the mixture of essential fatty acids (EFAs) was developed by using lipoxygenase (LOX) (EC 1.13.11.

Comparison of chitin and Amberlite IRA-938 for alpha-galactosidase immobilization.

Watermelon alpha-galactosidase (EC 3.2.1.

Encapsulation of insulin in chitosan-coated alginate beads: oral therapeutic peptide delivery.

Insulin was encapsulated in calcium alginate beads coated with chitosan. Its release from alginate-chitosan and alginate-chitosan-glutaraldehyde beads was studied in artificial gastric (pH 1.2) and intestinal (pH 7.