Weak promoters to drive selection marker expression: Improvement of cell line development process for therapeutic protein production in CHO-K1 cells.

Chinese Hamster Ovary cells have been widely used as host cells for production of recombinant therapeutic molecules. Cell line development is a decisive step, which must be carried out with an efficient process. In particular, degree of selection stringency is an important parameter for identification of rare, high-producing cell lines.

Fluorescence-based rapid detection of microbiological contaminants in water samples.

Microbiological contamination of process waters is a current issue for pharmaceutical industries. Traditional methods require several days to obtain results; therefore, rapid microbiological methods are widely requested to shorten time-to-result. Milliflex Quantum was developed for the rapid detection and enumeration of microorganisms in filterable samples.

Novel sample preparation method for molecular detection of Mollicutes in cell culture samples.

Research laboratories, raw materials and media suppliers as well as the biopharmaceutical industry face recurrent contamination with Mollicutes. Culture-based detection methods are very slow (28 days) and could ideally be replaced by nucleic acid testing (NAT) for rapid result. These methods are nonetheless hampered by their companion sample preparation methods.

Adenoviral-mediated skeletal muscle transcriptional targeting using chimeric tissue-specific promoters.

Background: Adenoviral vectors are promising tools to achieve skeletal muscle gene transfer for the treatment of peripheral ischemia. However, the use of ubiquitous viral promoters represents a major safety issue that could limit their use. Cellular regulatory sequences that allow strong and tissue-specific expression could circumvent this problem.