Quantitative Live Single-cell Mass Spectrometry with Spatial Evaluation by Three-Dimensional Holographic and Tomographic Laser Microscopy.

The locations and volumes of the contents of a single HepG2 cell were visualized under three-dimensional (3D) holographic and tomographic (HT) laser microscopy, colored by refractive index, not staining. After trapping the specific area of a target cell in a nanospray tip, quantification was performed by live single-cell mass spectrometry. Comparison of the HepG2 cells' before and after 3D-HT images allowed the inference of the precise volume and original location of the trapped cell contents.

Phase extraction in speckle interferometry by a circle fitting procedure in the complex plane.

In speckle interferometry (SI), temporal signals are amplitude- and frequency-modulated signals and exhibit a fluctuating background. Prior to phase computation, this background intensity must be eliminated. Here our approach is to build a complex signal from the raw one and to fit a circle through the points cloud representing its sampled values in the complex plane.

Snap-shot profilometry with the Empirical Mode Decomposition and a 3-layer color sensor.

Remote sensing finds more and more applications, from industrial control, to face recognition, not forgetting terrain surveying. This trend is well exemplified by fringe projection techniques, which enjoyed a considerable development in the recent years. In addition of high requirement in terms of measurement accuracy and spatial resolution, the end-users of full-field techniques show a growing interest for dynamic regimes.

The empirical mode decomposition: a must-have tool in speckle interferometry?

In a wider and wider range of research and engineering activities, there is a growing interest for full-field techniques, featuring nanometric sensitivities, and able to be addressed to dynamic behaviors characterization. Speckle interferometry (SI) techniques are acknowledged as good candidates to tackle this challenge. To get rid of the intrinsic correlation length limitation and simplify the un-wrapping step, a straightforward approach lies in the pixel history analysis.