Publications by authors named "Sebastian D Schuck"
Background: T-cell responses against dormancy-, resuscitation-, and reactivation-associated antigens of Mycobacterium tuberculosis are candidate biomarkers of latent infection in humans.
Methodology/principal Findings: We established an assay based on two rounds of in vitro restimulation and intracellular cytokine analysis that detects T-cell responses to antigens expressed during latent M. tuberculosis infection.
Multifunctional T cells expressing several cytokines in parallel are thought to play a crucial role in protection against different infections. To characterize T cell cytokine patterns associated with disease and protection in Mycobacterium tuberculosis infection we determined the expression of IFNgamma, IL-2, TNFalpha, and GM-CSF in T cell subpopulations from children with tuberculosis (TB) and healthy latently M. tuberculosis-infected children (LTBI) after short-term in vitro restimulation.
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- Human cytomegalovirus (CMV) significantly affects individuals with weakened immune systems, yet no effective vaccine exists.
- Identifying target proteins and T-cell epitopes is essential for creating a successful vaccine and for new techniques involving T-cell therapy.
- The study introduces a streamlined SPOT synthesis method to create and test all potential CD8 T-cell epitopes from the CMV proteome, leading to the discovery of many known and novel epitopes after testing over 9,000 peptides.