Ribosome Stalling of -Linked Glycoproteins in Cell-Free Extracts.

Ribosome display is a powerful method for selection and directed evolution of proteins expressed from combinatorial libraries. However, the ability to display proteins with complex post-translational modifications such as glycosylation is limited. To address this gap, we developed a set of complementary methods for producing stalled ribosome complexes that displayed asparagine-linked (-linked) glycoproteins in conformations amenable to downstream functional and glycostructural interrogation.