Neurogenomic divergence during speciation by reinforcement of mating behaviors in chorus frogs (Pseudacris).

Background: Species interactions can promote mating behavior divergence, particularly when these interactions are costly due to maladaptive hybridization. Selection against hybridization can indirectly cause evolution of reproductive isolation within species, a process termed cascade reinforcement. This process can drive incipient speciation by generating divergent selection pressures among populations that interact with different species assemblages.

Hierarchical Hybrid Enrichment: Multitiered Genomic Data Collection Across Evolutionary Scales, With Application to Chorus Frogs (Pseudacris).

Determining the optimal targets of genomic subsampling for phylogenomics, phylogeography, and population genomics remains a challenge for evolutionary biologists. Of the available methods for subsampling the genome, hybrid enrichment (sequence capture) has become one of the primary means of data collection for systematics, due to the flexibility and cost efficiency of this approach. Despite the utility of this method, information is lacking as to what genomic targets are most appropriate for addressing questions at different evolutionary scales.

Growth Dynamics and Survival of BT-1, an Important Model Organism for the Citrus Huanglongbing Pathogen " Liberibacter asiaticus".

is the only cultured member of its genus, which includes the devastating plant pathogen " Liberibacter asiaticus," associated with citrus greening/Huanglongbing (HLB). has a larger genome and greater metabolic flexibility than " Liberibacter asiaticus" and the other uncultured plant-pathogenic species, and it is currently the best model organism available for these pathogens. grows slowly and dies rapidly under current culture protocols and this extreme fastidiousness makes it challenging to study.

Anchored hybrid enrichment generated nuclear, plastid and mitochondrial markers resolve the Lepanthes horrida (Orchidaceae: Pleurothallidinae) species complex.

Phylogenetic relationships in species complexes and lineages derived from rapid diversifications are often challenging to resolve using morphology or standard DNA barcoding markers. The hyper-diverse genus Lepanthes from Neotropical cloud forest includes over 1200 species and many recent, explosive diversifications that have resulted in poorly supported nodes and morphological convergence across clades. Here, we assess the performance of 446 nuclear-plastid-mitochondrial markers derived from an anchored hybrid enrichment approach (AHE) coupled with coalescence- and species network-based inferences to resolve phylogenetic relationships and improve species recognition in the Lepanthes horrida species group.

Normal Vulvar Histology: Variation by Site.

Objective: The aim of the study was to assess the histology of normal vulvar skin with attention to anatomic location and epithelium type.

Materials And Methods: We performed a retrospective histologic review of 118 vulvar biopsies and excisions obtained between 2010 and 2014 with adjacent normal skin or mucosa. Exclusions included age younger than 18 years, vestibulectomy, labiaplasty, inflammatory dermatoses, and insufficient normal tissue for assessment.