Publications by authors named "Sean Gallagher"

Background/aim: Transgenic nude mice expressing green fluorescent protein (GFP), red fluorescent protein (RFP), or cyan fluorescent protein (CFP) were previously developed by our laboratory, AntiCancer Inc. In the present study, we demonstrate imaging of the GFP, RFP, or CFP nude mice with single-nanometer-tuning laser fluorescence excitation with a single instrument.

Materials And Methods: Female transgenic C57/B6 nude GFP, RFP, and CFP mice aged six weeks were used.

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  • Data shows that both ad hoc and planned percutaneous coronary intervention (PCI) for unprotected left main stem (uLMS) procedures have been studied, but outcomes for ad hoc cases were previously unclear.* -
  • Analysis of over 8,500 uLMS-PCI procedures revealed that ad hoc interventions resulted in higher rates of complications and adverse events, such as in-hospital death and major cardiac issues, compared to planned interventions.* -
  • The findings suggest that ad hoc uLMS-PCI is linked to worse in-hospital outcomes, indicating the need for careful procedural planning in such cases.*
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Background/aim: In vivo imaging with luciferase-luciferin has been limited by the inability to visualize the low emitted light, with the signal quantified only by photon counting using a cumbersome highly-cooled CCD camera in a dark room. In the present study, we demonstrate direct visualization of the luciferase-luciferin signal from an orthotopic lung cancer in a nude-mouse xenograft model with a sensitive low-light camera and optics.

Materials And Methods: Mouse Lewis-lung carcinoma cells expressing luciferase (LL/2-Luc2) were injected transcutaneously into the lung of a nude mouse.

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  • Chronic total occlusion (CTO) in coronary arteries poses significant challenges during percutaneous coronary interventions (PCI), often requiring advanced methods and tools like microcatheters.
  • The BIOMICS study tested a new microcatheter (BioMC) in 100 patients with ischemic symptoms undergoing CTO-PCI, focusing on its ability to successfully facilitate guide wire placement and monitoring for safety issues like cardiac death or myocardial infarction.
  • Results showed a 75% success rate in achieving the primary efficacy goal and only 2% incidence of severe safety events, indicating that the new microcatheter is both effective and safe in treating CTO.
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  • The study compares the effectiveness of two imaging techniques: green fluorescent protein (GFP) fluorescence and luciferase-luciferin imaging, using advanced detection methods in live mice.
  • Results showed that GFP provided more stable and intense signals over time compared to luciferase-luciferin, which significantly decreased in intensity after 20 minutes.
  • Additionally, GFP imaging required a much shorter exposure time (100 ms) compared to luciferase-luciferin imaging (30 seconds) to detect signals effectively.
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Background: Minor physical anomalies (MPAs) are congenital morphological abnormalities linked to disruptions of fetal development. MPAs are common in 22q11.2 deletion syndrome (22q11DS) and psychosis spectrum disorders (PS) and likely represent a disruption of early embryologic development that may help identify overlapping mechanisms linked to psychosis in these disorders.

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Automobile seat belts reduce the risk of injuries and fatalities resulting from a crash. As seat belts become more prevalent on large school buses, characterizing the capabilities of children to operate the unlatching mechanism of a seat belt is crucial to ensure the post-crash safety of young passengers. This study evaluated the strength capabilities of children and their abilities to unlatch a school bus seat belt when a school bus is in both the upright and rolled-over orientations.

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This study assesses the agreement of compressive and shear force estimates at the L5-S1 joint using inertial motion capture (IMC) within a musculoskeletal simulation model during manual lifting tasks, compared against a top-down optical motion capture (OMC)-based model. Thirty-six participants completed lifting and lowering tasks while wearing a modified Plug-in Gait marker set for the OMC and a full-body IMC set-up consisting of 17 sensors. The study focused on tasks with variable load weights, lifting heights, and trunk rotation angles.

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  • Pancreatic cancer has a low 5-year survival rate of 12%, highlighting the need for better mouse models to explore new treatments.
  • Researchers developed a precise orthotopic mouse model using human pancreatic cancer cells (Panc-1) tagged with green fluorescent protein (GFP), enabling non-invasive imaging of tumors in the pancreas.
  • The study found that non-invasive fluorescence imaging matched invasive laparotomy images, demonstrating the model’s effectiveness for future screening of pancreatic cancer therapies.
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The purpose of the present study was to determine the effects of obesity and biological sex on myostatin expression in humans and to examine the direct effects of myostatin, SMAD2, and SMAD3 on insulin signaling in primary human skeletal muscle cells (HSkMCs). For , 15 lean [body mass index (BMI): 22.1 ± 0.

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A prior experiment identified separate thought and feeling item groupings among items in measures of unhelpful thinking (ie, catastrophic thinking, kinesiophobia). This study sought to confirm the utility of separating these factors using a subset of selected items. One hundred and thirty-six adult patients visiting a musculoskeletal specialist completed the surveys.

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Background: Percutaneous coronary intervention (PCI) for coronary chronic total occlusion (CTO) has been performed for the improvement of symptoms and quality of life in patients with stable angina. The ORBITA study demonstrated the role of the placebo effect in contemporary PCI in non-CTO chronic coronary syndromes. However, the benefit of CTO PCI beyond that of a placebo has not been demonstrated.

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Background: Neuropsychiatric disorders are common in 22q11.2 Deletion Syndrome (22q11DS) with about 25% of affected individuals developing schizophrenia spectrum disorders by young adulthood. Longitudinal evaluation of psychosis spectrum features and neurocognition can establish developmental trajectories and impact on functional outcome.

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Digital imaging is the method of choice for documentation and analysis of electrophoretic separations of protein and DNA. Digital images of gel electropherograms can be obtained rapidly using sCMOS and CCD-based cameras, and the images can be easily archived and analyzed using image analysis software. This overview defines important key terms and calculations for imaging, explains the capture process, reviews the range of technologies used for image capture, and provides an introduction to the software and methods used for one- and two-dimensional digital image analysis.

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Background: Percutaneous coronary intervention (PCI) is increasingly used as a treatment option for unprotected left main stem artery (unprotected left main stem percutaneous intervention) disease. However, whether patient outcomes have improved over time is uncertain.

Methods: Using the United Kingdom national PCI database, we studied all patients undergoing unprotected left main stem percutaneous intervention between 2009 and 2017.

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Out-of-hospital cardiac arrest (OHCA) affects 80,000 patients per year in the UK; despite improvements in care, survival to discharge remains lower than 10%. NHS England and several societies recommend all resuscitated OHCA patients be directly transferred to a cardiac arrest centre (CAC). However, evidence is limited that all patients benefit from transfer to a CAC, and there are significant organisational, logistic and financial implications associated with such change in policies.

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Out-of-hospital cardiac arrest (OHCA) is a major public health issue that poses significant challenges both in immediate management and long-term follow-up. Survivors of OHCA often experience a combination of complex medical, physical and psychological needs that have a significant impact on quality of life. Guidelines suggest a multi-dimensional follow-up to address both physical and non-physical domains for survivors.

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(1) Background: The objectives of this systematic review were to (i) summarize the results of studies evaluating the reliability of observational ergonomics exposure assessment tools addressing exposure to physical risk factors associated with upper extremity musculoskeletal disorders (MSDs), and (ii) identify best practices for assessing the reliability of new observational exposure assessment tools. (2) Methods: A broad search was conducted in March 2020 of four academic databases: PubMed, Science Direct, Ergonomic Abstracts, and Web of Science. Articles were systematically excluded by removing redundant articles, examining titles and abstracts, assessing relevance to physical ergonomics and the upper extremities, and article type.

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In this work, a novel mock circulatory loop (MCL) is presented that is capable of simulating both healthy cardiac function and Heart Failure with preserved Ejection Fraction (HFpEF). This MCL differs from others presented in the literature as it features two independently actuated heart chambers, representing the left atrium and the left ventricle. This is an important improvement over other designs as it allows for potential HFpEF treatments to be examined, not just in relation to their effect on the left ventricle but also on the left atrium.

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With the increasing number and complexity of interventional cardiology procedures, there is the potential for higher occupational radiation doses to the interventionists. In order to reduce the radiation exposure to interventionists, a number of different radiation protection measures can be implemented; the most common of which being personal protective equipment in the form of a lead-equivalent apron. However, significant development has been achieved with mobile lead equivalent radiation protection devices, which provide enhanced radiation protection without the requirement of being directly worn by staff.

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  • Personalized prognosis is crucial for deciding between PCI and CABG in patients with three-vessel disease, as shown in a study comparing local heart team decisions with individualized mortality predictions.
  • * The study evaluated SYNTAX score II and SYNTAX score 2020 in 200 patients, revealing discrepancies where many recommended CABG patients actually received PCI instead.
  • * Although risk models provided significant insights, notable deviations from recommendations in revascularization methods were observed in the initial cohort screened in the Multivessel TALENT trial.*
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Introduction: There is increasing evidence supporting the use of intracoronary imaging to optimize the outcomes of percutaneous coronary intervention (PCI). However, there are no studies examining the impact of imaging on PCI outcomes in cases utilising rotational atherectomy (RA-PCI). Our study examines the determinants and outcomes of using intracoronary imaging in RA-PCI cases including 12-month mortality.

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