Publications by authors named "Sean Beecroft"
We undertook a comprehensive proteogenomic characterization of 95 prospectively collected endometrial carcinomas, comprising 83 endometrioid and 12 serous tumors. This analysis revealed possible new consequences of perturbations to the p53 and Wnt/β-catenin pathways, identified a potential role for circRNAs in the epithelial-mesenchymal transition, and provided new information about proteomic markers of clinical and genomic tumor subgroups, including relationships to known druggable pathways. An extensive genome-wide acetylation survey yielded insights into regulatory mechanisms linking Wnt signaling and histone acetylation.
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- Prostate cancer is a major cause of cancer-related deaths in men, and current screening methods like PSA tests can lead to false positives and unnecessary procedures.
- Research into glycans—sugar molecules attached to proteins—has gained attention as they could provide a more reliable method of diagnosing prostate cancer.
- The study used advanced techniques to analyze these glycans from prostate cell lines and human serum, revealing notable differences in certain glycans present in the serum samples of prostate cancer patients.
The investigation of site-specific glycosylation is essential for further understanding the many biological roles that glycoproteins play; however, existing methods for characterizing site-specific glycosylation either are slow or yield incomplete information. Mass spectrometry (MS) is being applied to investigate site-specific glycosylation with bottom-up proteomic type strategies. When using these approaches, tandem mass spectrometry techniques are often essential to verify glycopeptide composition, minimize false positives, and investigate structure.
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