Publications by authors named "Sean Baker"
(turkey) coexpresses distinct hemoglobin (Hb) isoforms, Hb αβ (HbA) and αβ (HbD), at a ratio of ∼3:1 (HbA:HbD). Herein, the reactivities of HbA and HbD were investigated in their native and free fatty acid (FFA)-modified states. Results indicated that HbD displays elevated autoxidation () and an increased propensity to oxidize lipids in its reduced (oxy) and oxidized (met) forms.
View Article and Find Full Text PDFRapid Lentiviral Vector Producer Cell Line Generation Using a Single DNA Construct.
Mol Ther Methods Clin Dev
December 2020
Article Synopsis
- The study presents a method to create stable suspension cell lines from 293T cells that produce lentiviral vectors using a single DNA construct, which simplifies the production process compared to the traditional method involving multiple plasmids.
- These new cell lines can achieve high yields comparable to those from transient transfection and are designed to be easily scaled up in bioreactors, promoting efficient large-scale production.
- By streamlining the production process and improving stability and yield, this approach aims to enhance patient access to therapies developed using lentiviral vectors.
Objective: To compare the biomechanical properties of clamp rod internal fixation (CRIF)/rod and LC-DCP/rod constructs in a canine femoral gap model.
Study Design: Cadaveric biomechanical study.
Sample Population: Canine femora (n = 10 pair).
DNA mismatch repair (DMR) functions to maintain genome stability. Prokaryotic and eukaryotic cells deficient in DMR show a microsatellite instability (MSI) phenotype characterized by repeat length alterations at microsatellite sequences. Mice deficient in Pms2, a mammalian homolog of bacterial mutL, develop cancer and display MSI in all tissues examined, including the male germ line where a frequency of approximately 10% was observed.
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