Isolation of a Potential Probiotic and Evaluation of Its Exopolysaccharide for Antioxidant and α-Glucosidase Inhibitory Activities.

The probiotic properties of ten lactic acid bacteria and antioxidant and α-glucosidase inhibitory activities of the exopolysaccharide (EPS) of the selected strain were investigated in this study. L010 was one of the most active strains across all the in tests. The cell-free supernatant (50 g/l) of L010 showed high levels of both α-glucosidase inhibitory activity (98.

Isolation of acid tolerant lactic acid bacteria and evaluation of α-glucosidase inhibitory activity.

In this study, lactic acid bacteria strains (LABs) were isolated from Korean traditional fermented food and examined as potential probiotics using in vitro methods. Ten LAB strains survived in de Man, Rogosa and Sharpe broth adjusted to pH 2.5 were tested for resistance to acidic conditions and bile, antimicrobial activity, and α-glucosidase inhibitory activity.

Complete genome sequence data of MBEL1397 isolated from kimchi.

MBEL1397(=KCTC14037BP) was isolated from kimchi, a traditional Korean fermented food, in Gangwon province, Republic of Korea. MBEL1397 is an acid-tolerant strain with antimicrobial activity and α-glucosidase inhibitory activity, which might be preliminary indications of its probiotic properties. Complete genome sequencing of MBEL1397 was performed using the PacBio RSII platform.

Cloning and functional verification of the Candida milleri HIS3 gene encoding imidazoleglycerol phosphate dehydratase.

The entire nucleotide sequence of the HIS3 gene encoding imidazoleglycerol phosphate dehydratase (IGPD) in yeast Candida milleri CBS 8195 was determined. Sequence analysis revealed an open-reading frame of C. milleri HIS3 that spans 678 bp, encodes 226 amino acids, and shares 80.