Antimicrobial resistance induction potential of grapefruit seed extract on multi-species biofilm of E. coli in food industry.

Biofilm formation in natural environments involving complex multi-structural arrangements hinders challenges in antimicrobial resistance. This study investigated the antimicrobial resistance potential of grapefruit seed extract (GSE) by examining the formation of mono-, dual-, and multi-species biofilms. We also explored the counterintuitive effect in response to GSE at various concentrations, including minimum inhibitory concentration (MIC) and sub-MIC (1/2 and 1/4 MIC).

Enhanced detection of Listeria monocytogenes using tetraethylenepentamine-functionalized magnetic nanoparticles and LAMP-CRISPR/Cas12a-based biosensor.

Background: Listeria monocytogenes is a pathogenic bacterium that can lead to severe illnesses, especially among vulnerable populations. Therefore, the development of rapid and sensitive detection methods is vital to prevent and manage foodborne diseases. In this study, we used tetraethylenepentamine (TEPA)-functionalized magnetic nanoparticles (MNPs) and a loop-mediated isothermal amplification (LAMP)-based CRISPR/Cas12a-based biosensor to concentrate and detect, respectively, L.

Point-of-Care Diagnostic System for Viable Species via Improved Propidium Monoazide and Recombinase Polymerase Amplification Based Nucleic Acid Lateral Flow.

species are prominent foodborne microbial pathogens transmitted through contaminated food or water and pose a significant threat to human health. Accurate and rapid point-of-care (POC) diagnosis is gaining attention in effectively preventing outbreaks of foodborne disease. However, the presence of dead bacteria can interfere with an accurate diagnosis, necessitating the development of methods for the rapid, simple, and efficient detection of viable bacteria only.

Naked-eye detection with loop-mediated isothermal amplification for subsp. in agricultural products.

Unlabelled: causing soft-rot disease requires on-site detection before the distribution of agricultural products. Loop-mediated isothermal amplification (LAMP), which is resistant to food inhibitors, is known for its high detection sensitivity for pathogens and when coupled with lateral flow immunoassay (LFA) enables visualizations. For detection of soft-rot disease, we developed a LAMP-LFA system targeting 16S ribosomal RNA, a partial sequence gene of subsp.

Quantitative risk assessment of foodborne illness by estimating cooking effect on eggs from retail markets.

In this study, we performed a quantitative microbial risk assessment (QMRA) of through intake of egg consumption after cooking (dry-heat, moist-heat, and raw consumption). Egg samples (n = 201) from retail markets were analyzed for the presence of . In addition, temperature and time were investigated during egg transit, storage, and display.

Diverse methods of reducing and confirming false-positive results of loop-mediated isothermal amplification assays: A review.

Loop-mediated isothermal amplification (LAMP), a rapid and sensitive isothermal nucleic acid amplification method, is a promising alternative to other molecular amplification techniques due to its superior specificity and sensitivity. However, due to primer dimerization, LAMP results in nonspecific and nontemplate amplification. And during the amplification confirmation process, there is carry-over contamination.

A review of mechanism analysis methods in multi-species biofilm of foodborne pathogens.

Biofilms are an aggregation of microorganisms that have high resistance to antimicrobial agents. In the food industry, it has been widely studied that foodborne pathogens on both food surfaces and food-contact surfaces can form biofilms thereby threatening the safety of the food. In the natural environment, multi-species biofilms formed by more than two different microorganisms are abundant.

Risk Assessment in Poultry Meat from Farm to Consumer in Korea.

This study predicted outbreak risk from eating cooked poultry in various methods. The incidence of in poultry meat and the environment from farm to home for consumption was investigated. To develop the predictive models, growth data were collected at 4-25 °C during storage and fitted with the Baranyi model.

Thermophilic helicase-dependent amplification-based CRISPR/Cas12a system: Detection of stx2 in Escherichia coli O157:H7 by controlling primer dimers.

Background: s: To overcome the limitation of polymerase chain reaction (PCR), isothermal amplification methods such as thermophilic helicase-dependent amplification (tHDA) have been developed. However, formation of primer dimer due to the single amplification temperature are major problems of tHDA. When cross-dimerization of forward and reverse primer occurred, false-positive results can be found on the lateral flow assay (LFA) which is one of the major detection methods widely used as a point of care diagnosis.

Combination of filtration and immunomagnetic separation based on real-time PCR to detect foodborne pathogens in fresh-cut apple.

Rapid detection methods require pre-enrichment culture in order to detect low levels of foodborne pathogens. To rapidly detect foodborne pathogens, enrichment culture processes could be replaced. Filtration and immunomagnetic separation methods have been identified to effectively concentrate and separate target pathogens from foods.

Antibacterial activities of polyphenols against foodborne pathogens and their application as antibacterial agents.

Polyphenols are secondary metabolites produced in higher plants. They are known to possess various functional properties in the human body. Polyphenols also exhibit antibacterial activities against foodborne pathogens.

A Review of Isothermal Amplification Methods and Food-Origin Inhibitors against Detecting Food-Borne Pathogens.

The isothermal amplification method, a molecular-based diagnostic technology, such as loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA), is widely used as an alternative to the time-consuming and labor-intensive culture-based detection method. However, food matrices or other compounds can inhibit molecular-based diagnostic technologies, causing reduced detection efficiencies, and false-negative results. These inhibitors originating from food are polysaccharides and polyphenolic compounds in berries, seafood, and vegetables.

Filtration-based LAMP-CRISPR/Cas12a system for the rapid, sensitive and visualized detection of Escherichia coli O157:H7.

Escherichia coli O157:H7 is a major cause of fresh vegetable-associated infections that can threaten human health. A method for rapidly detecting food-borne pathogens should be developed for safe food management. A clustered regularly interspaced short palindromic repeats (CRISPR)-based detection method has the potential to greatly advance biosensing technology through its high sensitivity and specificity.

Development of modified enrichment broth for short enrichment and recovery of filter-injured Salmonella Typhimurium.

The filter concentration method facilitates the rapid detection of foodborne pathogens. The filter concentration method lowered the limit of detection (LOD) of artificially inoculated cabbage with Salmonella Typhimurium; however, the procedure injured foodborne pathogens during filtering procedure. Thus, to detect injured pathogens under the detection limit, an enrichment broth promoting pathogen resuscitation and growth is required.

Enhancing safety and quality of shrimp by nanoparticles of sodium alginate-based edible coating containing grapefruit seed extract.

Edible coatings are safe and effective in extending the shelf life of foods. In this study, a nanoparticle-based edible coating solution was prepared, containing alginate as a coating agent and grapefruit seed extract as an antibacterial agent to improve the safety and quality of shrimp during storage. Shrimp coated with this formulation were maintained at 4°C for 8 days, and periodically analyzed for changes in sensory, chemical [total volatile basic nitrogen (TVB-N) and pH] and microbial parameters.

Development of enhanced selective media for detection of in oysters.

Unlabelled: This study was undertaken to develop enhanced selective media for detection of in oysters. Primarily, tryptic soy agar (TSA) was supplemented with 4.5-5% NaCl, 0.

Review of multi-species biofilm formation from foodborne pathogens: multi-species biofilms and removal methodology.

Multi-species biofilms are ubiquitous worldwide and are a concern in the food industry. Multi-species biofilms have a higher resistance to antimicrobial therapies than mono-species biofilms. In addition, multi-species biofilms can cause severe foodborne diseases.

Optimization of resuscitation-promoting broths for the revival of from a viable but nonculturable state.

This study was conducted to examine the effect of formulated resuscitation-promoting broths on the revival of viable but nonculturable induced by cold and starvation stresses. was incubated in artificial sea water at 4 °C for more than 8 months until this bacterium became undetectable, while retaining its intact cell count of more than 10 CFU/field over time. On day 250, .

Correction to: Electrochemical impedimetric biosensors for food safety.

[This corrects the article DOI: 10.1007/s10068-020-00776-w.].

Rapid detection of O157:H7 by a novel access with combination of improved sample preparation and real-time PCR.

Foodborne bacteria are typically present at very low concentrations in food. This study describes a quick and simple method for concentrating O157:H7 present in lettuce and cabbage, without microbial enrichment culture. This method involved reducing the extraction buffer and DNA elution volumes.

Electrochemical impedimetric biosensors for food safety.

Electrochemical impedimetric biosensors (EIBs) have a simple structure and can be used to rapidly and sensitively detect and measure hazards in food. EIBs detect and measure target molecules by transducing biochemical reactions on their surface to electrical signal outputs responding to a sinusoidal electrical signal input. Due to their structural simplicity and analytical sensitivity, EIBs are regarded as the most potent method of food hazard monitoring that can be implemented in the food supply chain.

Rapid and sensitive detection of E. coli O157:H7 and S. Typhimurium in iceberg lettuce and cabbage using filtration, DNA concentration, and qPCR without enrichment.

The outbreaks due to the low number of foodborne pathogens present in ready-to-eat products can be prevented by rapid and sensitive detection method. However, as a conventional detection method, it is impossible to monitor foodborne bacteria existing which is less than 50 cfu/25 g in a food. This study was designed to investigate the possibility of detecting 1 cfu in the short-term through filtration, DNA concentration, and qPCR.

Inactivation of Typhimurium and O157:H7 on black pepper powder using UV-C, UV-A and TiO coating.

This study was conducted to measure the inactivation characteristics of UVs and TiO against . Typhimurium and O157:H7 on black pepper powder. The sample was irradiated by UV-A and UV-C combined with TiO coating.

Power of Scanning Electron Microscopy and Energy Dispersive X-Ray Analysis in Rapid Microbial Detection and Identification at the Single Cell Level.

The demand for rapid, consistent and easy-to-use techniques for detecting and identifying pathogens in various areas, such as clinical diagnosis, the pharmaceutical industry, environmental science and food inspection, is very important. In this study, the reference strains of six food-borne pathogens, namely, Escherichia coli 0157: H7 ATCC 43890, Cronobacter sakazakii ATCC 29004, Salmonella Typhimurium ATCC 43971, Staphylococcus aureus KCCM 40050, Bacillus subtilis ATCC 14579, and Listeria monocytogenes ATCC 19115, were chosen for scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) analysis. In our study, the time-consuming sample preparation step for the microbial analysis under SEM was avoided, which makes this detection process notably rapid.

Optimization of Bacterial Concentration by Filtration for Rapid Detection of Foodborne Escherichia coli O157:H7 Using Real-Time PCR Without Microbial Culture Enrichment.

Escherichia coli O157:H7 is an important foodborne pathogen and has been implicated in numerous food poisoning outbreaks worldwide. Although several microbiological and molecular methods have been developed to detect E. coli O157:H7, the difficulty to rapidly detect low levels of the foodborne bacteria persists.