Factors Influencing Frequency of Depressive Experiences Among Married Working Women in South Korea.

: This study aimed to identify the factors influencing and predicting the frequency of depressive experiences among married working women in South Korea in the post-COVID-19 period (2022-2023). It examines how alterations in circumstances and the complex difficulties encountered by this demographic group may have shaped their depressive experiences. Through a comparative analysis of the group reporting depressive experiences and the group reporting no depressive experiences, the study delineates the factors influencing depressive experiences within the former group and the predictive factors within the latter group.

Design of a light and Ca switchable organic-peptide hybrid.

The design of organic-peptide hybrids has the potential to combine our vast knowledge of protein design with small molecule engineering to create hybrid structures with complex functions. Here, we describe the computational design of a photoswitchable Ca-binding organic-peptide hybrid. The designed molecule, designated Ca-binding switch (CaBS), combines an EF-hand motif from classical Ca-binding proteins such as calmodulin with a photoswitchable group that can be reversibly isomerized between a spiropyran (SP) and merocyanine (MC) state in response to different wavelengths of light.

Comprehensive analysis of Apigetrin's effects on liver cancer cells: Insights from bioinformatics, in vitro studies, and next-generation transcriptome sequencing.

Despite numerous attempts to understand the molecular mechanisms behind the development of liver cancer, it continues to pose a significant worldwide health challenge. Transcriptome sequencing, a powerful tool in molecular biology, has played a pivotal role in uncovering the intricate gene expression profiles underlying hepatocellular carcinoma (HCC). In the present study, we identified a total of 808 differentially expressed genes (DEGs), with 584 exhibiting downregulation, and 224 showing upregulation following apigetrin treatment.

()-Glucosciadopitysin, a New Biflavonoid Glycoside from the Leaves of and Osteogenic Activity of Bioflavonoids.

The leaves of have been used in treating freckles and effectively reducing cough and sputum in folk medicines. Recently, investigations into the correlation between ginkgo leaves and the proliferative activity of osteogenic differentiation have been conducted. However, bioactive compounds that enhance osteogenesis or exhibit osteoporosis prevention from have not been fully identified.

Transcriptome analysis revealed the genes and major pathways involved in prunetrin treated hepatocellular carcinoma cells.

Liver cancer represents a complex and severe ailment that poses tough challenges to global healthcare. Transcriptome sequencing plays a crucial role in enhancing our understanding of cancer biology and accelerating the development of more effective methods for cancer diagnosis and treatment. In the course of our current investigation, we identified a total of 1,149 differentially expressed genes (DEGs), encompassing 499 upregulated and 650 downregulated genes, subsequent to prunetrin (PUR) treatment.

Antioxidant and Anti-Inflammatory Properties of Conceivable Compounds from Leaf Extract on RAW264.7 Cells.

Background/objectives: is a medicinal plant, but the scientific basis is still unclear. This study thoroughly investigated phenols from extract (GLE) to determine their potential as anti-inflammatory and antioxidant agents.

Methods: High-performance liquid chromatography (HPLC) and mass spectrometry (MS) were used to analyze the compounds in GLE.

Artificial intelligence algorithm for neoplastic cell percentage estimation and its application to copy number variation in urinary tract cancer.

Background: Bladder cancer is characterized by frequent mutations, which provide potential therapeutic targets for most patients. The effectiveness of emerging personalized therapies depends on an accurate molecular diagnosis, for which the accurate estimation of the neoplastic cell percentage (NCP) is a crucial initial step. However, the established method for determining the NCP, manual counting by a pathologist, is time-consuming and not easily executable.

Binding affinity screening of polyphenolic compounds in Stachys affinis extract (SAE) for their potential antioxidant and anti-inflammatory effects.

Free radical is a marker in various inflammatory diseases. The antioxidant effect protects us from this damage, which also plays an essential role in preventing inflammation. Inflammation protects the body from biological stimuli, and pro-inflammatory mediators are negatively affected in the immune system.

Primary Metabolic Response of to Simulated Specialist Herbivory under Elevated CO Conditions.

This study explores how elevated carbon dioxide (CO) levels affects the growth and defense mechanisms of plants. We focused on Bunge (Aristolochiaceae), a wild plant that exhibits growth reduction under elevated CO in the previous study. The plant has Gray (Papilionidae) as a specialist herbivore.

Molecular networking and computational NMR analyses uncover six polyketide-terpene hybrids from termite-associated Xylaria isolates.

Fungi constitute the Earth's second most diverse kingdom, however only a small percentage of these have been thoroughly examined and categorized for their secondary metabolites, which still limits our understanding of the ecological chemical and pharmacological potential of fungi. In this study, we explored members of the co-evolved termite-associated fungal genus Xylaria and identified a family of highly oxygenated polyketide-terpene hybrid natural products using an MS/MS molecular networking-based dereplication approach. Overall, we isolated six no yet reported xylasporin derivatives, of which xylasporin A (1) features a rare cyclic-carbonate moiety.

Metabolic relationships between marine red algae and algae-associated bacteria.

Unlabelled: Mutualistic interactions between marine phototrophs and associated bacteria are an important strategy for their successful survival in the ocean, but little is known about their metabolic relationships. Here, bacterial communities in the algal sphere (AS) and bulk solution (BS) of nine marine red algal cultures were analyzed, and and were identified significantly more abundantly in AS than in BS. The metabolic features of RMAR6-6 (isolated and genome-sequenced), MAG 12 (obtained by metagenomic sequencing), and a marine red alga, CCMP1328 (from GenBank), were analyzed bioinformatically.

Investigation of prunetrin induced G2/M cell cycle arrest and apoptosis via Akt/mTOR/MAPK pathways in hepatocellular carcinoma cells.

Hepatocellular carcinoma (HCC) stands as a leading cause of mortality, and despite recent advancements in the overall survival rates, the prognosis remains dismal. Prunetin 4-O-glucoside (Prunetrin or PUR), an active compound derived from Prunus sp., was explored for its impact on HepG2 and Huh7 cells.

Potential Antioxidant and Anti-Inflammatory Properties of Polyphenolic Compounds from Extract.

is a medicinal plant that has been used due to its beneficial properties. However, extensive information regarding its therapeutic potential is scarce in the scientific literature. The antioxidant and anti-inflammatory potential of polyphenols derived from the extracts (CJE) was systematically analyzed.

IFITM3-mediated activation of TRAF6/MAPK/AP-1 pathways induces acquired TKI resistance in clear cell renal cell carcinoma.

Purpose: Vascular endothelial growth factor tyrosine kinase inhibitors (TKIs) have been the standard of care for advanced and metastatic clear cell renal cell carcinoma (ccRCC). However, the therapeutic effect of TKI monotherapy remains unsatisfactory given the high rates of acquired resistance to TKI therapy despite favorable initial tumor response.

Materials And Methods: To define the TKI-resistance mechanism and identify new therapeutic target for TKI-resistant ccRCC, an integrative differential gene expression analysis was performed using acquired resistant cohort and a public dataset.

Pinuseldarone, a Clerodane-Type Diterpene from Needles and Phytochemicals as Novel Agents for Regulating Brown Adipogenesis and Thermogenesis.

Phytochemical investigation of the MeOH extract of needles led to the isolation and identification of a new clerodane-type diterpene, pinuseldarone (), along with a known flavonoid, 5,4'-dihydroxy-3,7,8-trimethoxy-6-C-methylflavone (), through HPLC purification. The structure of the new compound was elucidated using spectroscopic methods, including 1D and 2D NMR, as well as HRESIMS. Its absolute configuration was established through NOESY analysis and computational methods, including electronic circular dichroism (ECD) calculations and gauge-including atomic orbital NMR chemical shift calculations, followed by DP4+ probability analysis.

Antiviral Effects of Secondary Metabolites from Leaves against the Pseudotyped Virus of SARS-CoV-2 Omicron.

holds a longstanding place in traditional herbal medicine, primarily utilized for addressing skin infections, acting as antipyretics, diuretics, and purgatives. In this study, our primary objective was to investigate the secondary metabolites present in leaves, with the aim of pinpointing natural compounds exhibiting potential antiviral activities. Five secondary metabolites (-), including an auronol glycoside (), two coumarins ( and ), a chromane () and a gallotannin (), were isolated from leaves.