The prevalence of visual axis opacification in the Swedish Pediatric Cataract Register.

Purpose: To report on the occurrence of postoperative visual axis opacification (VAO) in children younger than 5 years of age operated for cataract in Sweden, and to analyse correlations with age at surgery and surgical method.

Methods: Data were derived from the Swedish Pediatric Cataract Register (PECARE). All children operated on between 1 January 2007 and 31 December 2020 were included.

Study of the endogenous lectins of the human platelet plasma membrane.

Plasma membranes isolated from human blood platelets and human intact platelets, either fresh or previously fixed, were investigated for their possible content in intrinsic sugar-binding proteins (lectins) which can specifically bind monosaccharides such as fucose, mannose, and galactose. Light and heavy fractions of platelet plasma membrane and intact platelets fixed with 2% formaldehyde and suspended in Tyrode's buffer pH 7.0 were incubated with either L-(1-3H)-fucose, D-(1-3H)-mannose or D-(1-3H)-galactose.

Changes in the organization of membrane lipids during human platelet activation. Study by fluorescent and freeze-fracture cytochemistry.

Modifications in the membrane lipid organization of human platelets activated with different agents (adenosine 5'-diphosphate, thrombin, collagen type I, and monosaccharides such as fucose, mannose, and galactose) were analyzed in vitro by using three lipid markers. Cholesterol was detected upon interaction with filipin, the anionic phospholipids were reacted with polymyxin B, and alterations in the degree of lipid packing were evaluated with the lipophilic fluorescent probe merocyanine 540, which reportedly inserts into bilayer domains whose lipids are more disordered. Filipin-sterol complexes and polymyxin B-anionic phospholipid complexes form characteristic membrane deformations which were examined in freeze-fracture preparations, whereas the merocyanine 540 binding to platelet membrane was recorded by fluorescent microscopy.