Properties of packings and dispersions of superellipse sector particles.

Superellipse sector particles (SeSPs) are segments of superelliptical curves that form a tunable set of hard-particle shapes for granular and colloidal systems. SeSPs allow for continuous parametrization of corner sharpness, aspect ratio, and particle curvature; rods, circles, rectangles, and staples are examples of shapes SeSPs can model. We compare three computational processes: pair-wise Monte Carlo simulations that explore particle-particle geometric constraints, Monte Carlo simulations that reveal how these geometric constraints play out over dispersions of many particles, and Molecular Dynamics simulations that form random loose and close packings.

Development and large-scale production of human milk fat analog by fermentation of microalgae.

Background: Human milk contains a complex mixture of triacylglycerols (TAG), making it challenging to recreate using common ingredients.

Objective: The study aimed to develop an innovative fermentation technique to produce essential human milk TAG, effectively tackling a significant hurdle in infant nutrition.

Method: An in-depth analysis of the literature has been conducted to identify the specific TAG to be targeted.

Improving models for student retention and graduation using Markov chains.

Graduation rates are a key measure of the long-term efficacy of academic interventions. However, challenges to using traditional estimates of graduation rates for underrepresented students include inherently small sample sizes and high data requirements. Here, we show that a Markov model increases confidence and reduces biases in estimated graduation rates for underrepresented minority and first-generation students.

Excluded area of superellipse sector particles.

Superellipse sector particles (SeSPs) are segments of superelliptical curves that form a tunable set of hard-particle shapes for granular and colloidal systems. SeSPs allow for continuous parametrization of corner sharpness, aspect ratio, and particle curvature; rods, circles, rectangles, and staples are examples of shapes SeSPs can model. We investigate the space of allowable (nonoverlapping) configurations of two SeSPs, which depends on both the center-of-mass separation and relative orientation.

Black Soldier Fly Larvae Rearrange under Compression.

Thousands of black soldier larvae hatch simultaneously from eggs laid within rotting vegetation or animal carcasses. Over the next few weeks, they grow while compressed by both their surroundings and each other. When compressed, these larvae rearrange to reduce the forces upon them.

Elephant trunks form joints to squeeze together small objects.

Wild African elephants are voracious eaters, consuming 180 g of food per minute. One of their methods for eating at this speed is to sweep food into a pile and then pick it up. In this combined experimental and theoretical study, we elucidate the elephant's unique method of picking up a pile of food by compressing it with its trunk.

Delayed Flowering in Bamboo: Evidence from Fargesia qinlingensis in the Qinling Mountains of China.

Gregarious flowering of bamboo species impacts ecosystem properties and conservation, but documentation of these periodic events is difficult. Here, we compare the characteristics of flowering sites and un-flowered patches of an arrow bamboo (Fargesia qinlingensis) in the Qinling Mountains, China, over a 5-year period (2003-2007) after a mast flowering event (2003). We examined flowering culm and seedling characteristics in relation to questions regarding the evolution of delayed flowering.

Beta diversity determinants in Badagongshan, a subtropical forest in central China.

Niche and neutral theories emphasize different processes contributing to the maintenance of species diversity. In this study, we calculated the local contribution to beta diversity (LCBD) of every cell, using variation partitioning in combination with spatial distance and environmental variables of the 25-ha Badagongshan plot (BDGS), to determine the contribution of environmentally-related variation versus pure spatial variation. We used topography and soil characteristics as environmental variables, distance-based Moran's eigenvectors maps (dbMEM) to describe spatial relationships among cells and redundancy analysis (RDA) to apportion the variation in beta diversity into three components: pure environmental, spatially-structured environmental, and pure spatial.

16S and 23S plastid rDNA phylogenies of Prototheca species and their auxanographic phenotypes.

Because algae have become more accepted as sources of human nutrition, phylogenetic analysis can help resolve the taxonomy of taxa that have not been well studied. This can help establish algal evolutionary relationships. Here, we compare Auxenochlorella protothecoides and 23 strains of Prototheca based on their complete 16S and partial 23S plastid rDNA sequences along with nutrient utilization (auxanographic) profiles.

Safety evaluation of oleic-rich triglyceride oil produced by a heterotrophic microalgal fermentation process.

Numbers of macro- and microalgae have been used as food sources in various cultures for centuries. Several microalgae are currently being developed as modern food ingredients. The dietary safety of oleic-rich microalgal oil produced using a heterotrophic fermentation process was assessed in a 13-week feeding trial in rats with genotoxic potential evaluated using in vitro and in vivo assays.

Bioinformatics analyses reveal age-specific neuroimmune modulation as a target for treatment of high ethanol drinking.

Background: Use of in silico bioinformatics analyses has led to important leads in the complex nature of alcoholism at the genomic, epigenomic, and proteomic level, but has not previously been successfully translated to the development of effective pharmacotherapies. In this study, a bioinformatics approach led to the discovery of neuroimmune pathways as an age-specific druggable target. Minocycline, a neuroimmune modulator, reduced high ethanol (EtOH) drinking in adult, but not adolescent, mice as predicted a priori.

Entangled granular media.

We study the geometrically induced cohesion of ensembles of granular "u particles" that mechanically entangle through particle interpenetration. We vary the length-to-width ratio l/w of the u particles and form them into freestanding vertical columns. In a laboratory experiment, we monitor the response of the columns to sinusoidal vibration (with peak acceleration Γ).

Long-term population demography of Trillium recurvatum on loess bluffs in western Tennessee, USA.

Background And Aims: Understanding the demography of long-lived clonal herbs, with their extreme modularity, requires knowledge of both their short- and long-term survival and ramet growth patterns. The primary objective of this study was to understand the dynamics of a clonal forest herb, Trillium recurvatum, by examining temporal and small-scale demographic patterns. We hypothesized: (i) there would be more variability in the juvenile age class compared with non-flowering adult and flowering adult classes due to year-to-year fluctuations in recruitment; (ii) rates of population growth (λ) and increase (r) would be highest in non-flowering ramets due to a combination of transitions from the juvenile stage and reversions from flowering adults; and (iii) inter-ramet distances would be most variable between flowering and juvenile ramets due to a combination of clonal growth, seed dispersal by ants and ramet death over time.

Analysis of us-preexposure effects in appetitive conditioning.

In two experiments, rats received preexposure to one type of food followed by autoshaping in which presentation of one lever was associated with the preexposed food, and presentation of another lever with a novel type of food pellet. In both it was found that acquisition of the leverpress response occurred more readily on the lever associated with the novel food. This example of the US (unconditioned stimulus) preexposure effect is not to be explained in terms of the development of competing responses during preexposure.

Power-law flow statistics in anisometric (wedge) hoppers.

We find the probability for N particles to exit an anisometric (having unequal dimensions) hopper before jamming to have a broad power-law decay with exponent α = -2, in marked contrast to the exponential decay seen in hoppers with symmetric apertures. The transition from exponential to power law is explained by amodel that assumes particle motion is correlated over a distinct length scale. Hoppers with lengths larger than this length are modeled as a series of adjacent, statistically independent "cells.

Molecular factors affecting the accumulation of recombinant proteins in the Chlamydomonas reinhardtii chloroplast.

In an effort to develop microalgae as a robust system for the production of valuable proteins, we analyzed some of the factors affecting recombinant protein expression in the chloroplast of the green alga Chlamydomonas reinhardtii. We monitored mRNA accumulation, protein synthesis, and protein turnover for three codon-optimized transgenes including GFP, bacterial luciferase, and a large single chain antibody. GFP and luciferase proteins were quite stable, while the antibody was less so.

Column collapse of granular rods.

We investigate the collapse of granular rodpiles as a function of particle (length/diameter) and pile (height/radius) aspect ratio. We find that, for all particle aspect ratios below 24, there exists a critical height Hl below which the pile never collapses, maintaining its initial shape as a solid, and a second height Hu above which the pile always collapses. Intermediate heights between Hl and Hu collapse with a probability that increases linearly with increasing height.

Safety evaluation of a high-lipid algal biomass from Chlorella protothecoides.

Chlorella are traditionally freshwater green algae that have been evaluated for dietary purposes because of their nutritional value. This study investigates the safety of Chlorella protothecoides in a 28-day study. Sprague-Dawley rats were administered 0 (control), 2.

Jamming of three-dimensional prolate granular materials.

We have found that the ability of long thin rods to jam into a solidlike state in response to a local perturbation depends upon both the particle aspect ratio and the container size. The dynamic phase diagram in this parameter space reveals a broad transition region separating granular stick-slip and solidlike behavior. In this transition region the pile displays both solid and stick-slip behavior.

Contribution of 5'- and 3'-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes.

Expression of chloroplast genes is primarily regulated posttranscriptionally, and a number of RNA elements, found in either the 5'- or 3'-untranslated regions (UTRs) of plastid mRNAs, that impact gene expression have been identified. Complex regulatory and feedback mechanisms influence both translation and protein accumulation, making assignment of roles for specific RNA elements difficult. To identify specific contributions made by various UTRs on translation of plastid mRNAs, we used a heterologous gfp reporter gene that is fused combinatorially to chloroplast 5'- and 3'-UTRs.

Recent developments in the production of human therapeutic proteins in eukaryotic algae.

Antibody-based therapeutics have had great success over the last few years, and continue to be one of the fastest growing sectors of drug development. The efficacy and specificity of antibody-based drugs makes them ideal candidates for new drug development, but the specificity of these drugs comes from their complexity, and this complexity makes antibodies very expensive to produce. To address this problem, the authors have developed a system for the expression of recombinant proteins using the unicellular eukaryotic green algae, Chlamydomonas reinhardtii.