A comprehensive epidemiological approach documenting an outbreak of H5N1 highly pathogenic avian influenza virus clade 2.3.4.4b among gulls, terns, and harbor seals in the Northeastern Pacific.

Highly pathogenic avian influenza viruses (HPAIV) H5N1 clade 2.3.4.

Correction: Non-breeding changes in at-sea distribution and abundance of the threatened marbled murrelet (Brachyramphus marmoratus) in a portion of its range exhibiting long-term breeding season declines.

[This corrects the article DOI: 10.1371/journal.pone.

Baseline health parameters of rhinoceros auklets () using serum protein electrophoresis, acute phase proteins, and biochemistry.

Clinical metrics of baseline health in sentinel seabird species can offer insight into marine ecosystem dynamics, individual and population health, and assist in wildlife rehabilitation and conservation efforts. Protein electrophoresis is useful for detecting changes in acute phase proteins and immunoglobulin levels that may indicate subtle inflammatory responses and/or infectious disease. Serum biochemistry can highlight nutritional status, metabolic derangements, and organ injury and function.

Non-breeding changes in at-sea distribution and abundance of the threatened marbled murrelet (Brachyramphus marmoratus) in a portion of its range exhibiting long-term breeding season declines.

The marbled murrelet (Brachyramphus marmoratus) is classified as a threatened species under the US Endangered Species Act in Washington, Oregon, and California USA due to population declines, loss of breeding habitat, and other factors. To date, population assessments have focused on breeding season at-sea surveys. Consequently, there is little information on this species' distribution, abundance, and population trends during the non-breeding season, when murrelets are found exclusively in the marine environment.

Geolocator tagging links distributions in the non-breeding season to population genetic structure in a sentinel North Pacific seabird.

We tested the hypothesis that segregation in wintering areas is associated with population differentiation in a sentinel North Pacific seabird, the rhinoceros auklet (Cerorhinca monocerata). We collected tissue samples for genetic analyses on five breeding colonies in the western Pacific Ocean (Japan) and on 13 colonies in the eastern Pacific Ocean (California to Alaska), and deployed light-level geolocator tags on 12 eastern Pacific colonies to delineate wintering areas. Geolocator tags were deployed previously on one colony in Japan.

Detection of Bisgaard Taxon 40 in Rhinoceros Auklets ( Cerorhinca monocerata) with Pneumonia and Septicemia from a Mortality Event in Washington, USA.

We isolated Bisgaard taxon 40 from Rhinoceros Auklets ( Cerorhinca monocerata) with pneumonia and septicemia from Washington, US, found dead in 2016. Previously isolated only from the respiratory tract of a gull (Laridae), little is known about its pathogenic potential and whether it acts as a primary or opportunistic pathogen.

Two forage fishes as potential conduits for the vertical transfer of microfibres in Northeastern Pacific Ocean food webs.

We assessed the potential role played by two vital Northeastern Pacific Ocean forage fishes, the Pacific sand lance (Ammodytes personatus) and Pacific herring (Clupea pallasii), as conduits for the vertical transfer of microfibres in food webs. We quantified the number of microfibres found in the stomachs of 734 sand lance and 205 herring that had been captured by an abundant seabird, the rhinoceros auklet (Cerorhinca monocerata). Sampling took place on six widely-dispersed breeding colonies in British Columbia, Canada, and Washington State, USA, over one to eight years.

Will the California Current lose its nesting Tufted Puffins?

Tufted Puffin () populations have experienced dramatic declines since the mid-19th century along the southern portion of the species range, leading citizen groups to petition the United States Fish and Wildlife Service (USFWS) to list the species as endangered in the contiguous US. While there remains no consensus on the mechanisms driving these trends, population decreases in the California Current Large Marine Ecosystem suggest climate-related factors, and in particular the indirect influence of sea-surface temperature on puffin prey. Here, we use three species distribution models (SDMs) to evaluate projected shifts in habitat suitable for Tufted Puffin nesting for the year 2050 under two future Intergovernmental Panel on Climate Change (IPCC) emission scenarios.

Considering Spatial Scale and Reproductive Consequences of Habitat Selection when Managing Grasslands for a Threatened Species.

Habitat selection that has fitness consequences has important implications for conservation activities. For example, habitat characteristics that influence nest success in birds can be manipulated to improve habitat quality with the goal of ultimately improving reproductive success. We examined habitat selection by the threatened streaked horned lark (Eremophila alpestris strigata) at both the breeding-site (territory) and nest-site scales.

Using citizen-science data to identify local hotspots of seabird occurrence.

Seabirds have been identified and used as indicators of ecosystem processes such as climate change and human activity in nearshore ecosystems around the globe. Temporal and spatial trends have been documented at large spatial scales, but few studies have examined more localized patterns of spatiotemporal variation, by species or functional group. In this paper, we apply spatial occupancy models to assess the spatial patchiness and interannual trends of 18 seabird species in the Puget Sound region (Washington State, USA).

Assessing ecological correlates of marine bird declines to inform marine conservation.

Identifying drivers of ecosystem change in large marine ecosystems is central for their effective management and conservation. This is a sizable challenge, particularly in ecosystems transcending international borders, where monitoring and conservation of long-range migratory species and their habitats are logistically and financially problematic. Here, using tools borrowed from epidemiology, we elucidated common drivers underlying species declines within a marine ecosystem, much in the way epidemiological analyses evaluate risk factors for negative health outcomes to better inform decisions.

Persistent organic pollutants in forage fish prey of rhinoceros auklets breeding in Puget Sound and the northern California Current.

Organochlorine contaminants in upper trophic-level consumers inhabiting Puget Sound are consistently higher than in those species inhabiting other west coast locations. We analyzed persistent organic pollutants (POPs) in the six most common fish prey of rhinoceros auklets breeding on Protection Island (Puget Sound), Tatoosh Island (WA coast), and Destruction Island (WA coast). Wet-weight concentrations of POPs ranged widely (PCBs: 1.

Effects of elemental composition on the incorporation of dietary nitrogen and carbon isotopic signatures in an omnivorous songbird.

The use of stable isotopes to infer diet requires quantifying the relationship between diet and tissues and, in particular, knowing of how quickly isotopes turnover in different tissues and how isotopic concentrations of different food components change (discriminate) when incorporated into consumer tissues. We used feeding trials with wild-caught yellow-rumped warblers (Dendroica coronata) to determine delta15N and delta13C turnover rates for blood, delta15N and delta13C diet-tissue discrimination factors, and diet-tissue relationships for blood and feathers. After 3 weeks on a common diet, 36 warblers were assigned to one of four diets differing in the relative proportion of fruit and insects.

Cold temperature increases winter fruit removal rate of a bird-dispersed shrub.

We tested the hypothesis that winter removal rates of fruits of wax myrtle, Myrica cerifera, are higher in colder winters. Over a 9-year period, we monitored M. cerifera fruit crops in 13 0.